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  • KD/KO Validated

STAU1 Polyclonal antibody

STAU1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14225-1-AP

Synonyms

STAU, STAU1



Tested Applications

Positive WB detected inhuman brain tissue, K-562 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14225-1-AP targets STAU1 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAU1 fusion protein Ag5464
Full Name staufen, RNA binding protein, homolog 1 (Drosophila)
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC050432
Gene Symbol STAU1
Gene ID (NCBI) 6780
RRIDAB_2302744
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. There are two isoforms of STAU1 protein and the molecular weight of two isoforms are repectively 63 kDa and 55 kDa

Protocols

Product Specific Protocols
WB protocol for STAU1 antibody 14225-1-APDownload protocol
IHC protocol for STAU1 antibody 14225-1-APDownload protocol
IF protocol for STAU1 antibody 14225-1-APDownload protocol
IP protocol for STAU1 antibody 14225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

hiCLIP reveals the in vivo atlas of mRNA secondary structures recognized by Staufen 1.

Authors - Yoichiro Sugimoto
  • KD Validated
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
RIP

Mol Cell

Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation.

Authors - Shih-Han Lee
human,mouseWB,IHC,RIP

Nat Commun

Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk

Authors - Pingting Ying
humanWB

Neuron

Axonal Transport of TDP-43 mRNA Granules Is Impaired by ALS-Causing Mutations.

Authors - Nael H Alami
humanWB

Nucleic Acids Res

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Authors - Evan P Booy