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  • KD/KO Validated

Syntaxin 7 Polyclonal antibody

Syntaxin 7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12322-1-AP

Synonyms

STX7, syntaxin 7



Tested Applications

Positive WB detected inmouse spleen tissue, MDCK cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12322-1-AP targets Syntaxin 7 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity human, mouse, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntaxin 7 fusion protein Ag2975
Full Name syntaxin 7
Calculated Molecular Weight 261 aa, 30 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC011975
Gene Symbol STX7
Gene ID (NCBI) 8417
RRIDAB_2239979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 7 antibody 12322-1-APDownload protocol
IHC protocol for Syntaxin 7 antibody 12322-1-APDownload protocol
IF protocol for Syntaxin 7 antibody 12322-1-APDownload protocol
IP protocol for Syntaxin 7 antibody 12322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Proc Natl Acad Sci U S A

Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.

Authors - Bin Na-Ryum NR
ratWB,CoIP

J Cell Biol

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes

Authors - Min Li
  • KD Validated
humanWB

Biochem J

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.

Authors - Nazma Malik
humanWB

Cell Logist

Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins.

Authors - Carolina G Ortiz-Sandoval
human,mouseWB,IF

Neurobiol Dis

Syntaxin 7 modulates seizure activity in epilepsy

Authors - Junhong Wu
humanWB

J Biol Chem

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome

Authors - Hana Nůsková

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christian (Verified Customer) (10-12-2020)

The signal is strong and clean

  • Applications: Western Blot
  • Cell Tissue Type: b16f10