TBP Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 22246-1-AP

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Freight/Packing

Con: 28 μg/150 μl

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Species specificity:
human, mouse

Positive WB detected in:
Jurkat cells, COLO 320 cells, HeLa cells, mouse colon tissue, SKOV-3 cells

Positive IF detected in:
MCF-7 cells

Recommended dilution:
WB : 1:500-1:2000
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
TATA box binding protein

Calculated molecular weight:
338aa,38 kDa

Observed molecular weight:
mouse/rat 33-36 kDa and human 37-43kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TBP

Synonyms
GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID
Background

TBP, TATA box-binding protein, is a DNA-binding subunit of TFIID, which is a multisubunit complex essential for the expression of most protein-encoding genes. The well-conserved C-terminal domains of 180 aa is both necessary and sufficient for interaction with DNA and for assembly of the basal transcription apparatus.The N terminus of TBP modulates the DNA-binding activity of the C terminus of the protein. Once binds to the TATA box mediated by TBP, TFIID initiate the transcription step of the pre-initiation complex(PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This is a rabbit polyclonal antibody raised against part of N-terminal chain of human TBP, and specially recognize a ~46 kDa band.


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