Tie2 Polyclonal antibody

Tie2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 19157-1-AP


Angiopoietin 1 receptor, CD202B, hTIE2, p140 TEK, TEK, TIE 2, Tie2, VMCM, VMCM1

Tested Applications

Positive WB detected inmouse lung tissue, mouse liver tissue
Positive IP detected inmouse lung tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19157-1-AP is Tie2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tie2 fusion protein Ag13523
Full Name TEK tyrosine kinase, endothelial
Calculated molecular weight 1124 aa, 126 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC035514
Gene symbol TEK
Gene ID (NCBI) 7010
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. Tie2 acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene of Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.


Product Specific Protocols
WB protocol for Tie2 antibody 19157-1-APDownload protocol
IP protocol for Tie2 antibody 19157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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