CD71 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10084-2-AP
CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR
|Positive WB detected in||MCF7 cells, HeLa cells, K-562 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10084-2-AP targets CD71 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD71 fusion protein Ag0123|
|Full Name||transferrin receptor (p90, CD71)|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC001188|
|Gene ID (NCBI)||7037|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
|Product Specific Protocols|
|WB protocol for CD71 antibody 10084-2-AP||Download protocol|
|IHC protocol for CD71 antibody 10084-2-AP||Download protocol|
|IF protocol for CD71 antibody 10084-2-AP||Download protocol|
|IP protocol for CD71 antibody 10084-2-AP||Download protocol|
|Click here to view our Standard Protocols|
A novel iron(II) phenanthroline complex exhibits anticancer activity against TFR1-overexpressing esophageal squamous cell carcinoma cells through ROS accumulation and DNA damage.
Iron homeostasis and its disruption in mouse lung in iron deficiency and overload.
Liver metal levels and expression of genes related to iron homeostasis in rhesus monkeys after inhalational manganese exposure.
Biochem Biophys Res Commun
Myricetin ameliorates scopolamine-induced memory impairment in mice via inhibiting acetylcholinesterase and down-regulating brain iron.
FBW7 increases the chemosensitivity of pancreatic cancer cells to gemcitabine through upregulation of ENT1.
Dysregulated iron metabolism in the choroid plexus in fragile X-associated tremor/ataxia syndrome.