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CD71 Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 10084-2-AP

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Con: 28 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

CD71 Antibody WB MCF-7 cells 10084-2-AP

MCF-7 cells were subjected to SDS PAGE followed by western blot with 10084-2-AP (CD71 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CD71 Antibody IHC human placenta tissue 10084-2-AP

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:200 (under 10x lens).

CD71 Antibody IHC human placenta tissue 10084-2-AP

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:200 (under 40x lens).

CD71 Antibody IP HeLa cells 10084-2-AP

IP result of anti-CD71(IP:10084-2-AP, 4ug; Detection:10084-2-AP 1:500) with HeLa cells lysate 2400ug.

CD71 Antibody IF HeLa cells 10084-2-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 10084-2-AP (CD71 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, monkey, pig
Positive WB detected in:
MCF7 cells, HeLa cells, K-562 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD71 fusion protein Ag0123
Full name:
transferrin receptor (p90, CD71)
Calculated molecular weight:
85 kDa
Observed molecular weight:
90 kDa
GenBank accession number:
BC001188
Gene ID (NCBI):
7037
Gene symbol
TFRC
Synonyms
CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

Background

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.


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