CD71 Polyclonal antibody
CD71 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10084-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1080 cells, K-562 cells, HeLa cells, MCF-7 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 14 publications below |
| IHC | See 6 publications below |
| IF | See 1 publications below |
Product Information
10084-2-AP targets CD71 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, monkey, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD71 fusion protein Ag0123 |
| Full Name | transferrin receptor (p90, CD71) |
| Calculated molecular weight | 85 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC001188 |
| Gene symbol | TFRC |
| Gene ID (NCBI) | 7037 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD71 antibody 10084-2-AP | Download protocol |
| IHC protocol for CD71 antibody 10084-2-AP | Download protocol |
| IF protocol for CD71 antibody 10084-2-AP | Download protocol |
| IP protocol for CD71 antibody 10084-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol A novel iron(II) phenanthroline complex exhibits anticancer activity against TFR1-overexpressing esophageal squamous cell carcinoma cells through ROS accumulation and DNA damage. | ||
Exp Physiol Iron homeostasis and its disruption in mouse lung in iron deficiency and overload. | ||
Data Brief Liver metal levels and expression of genes related to iron homeostasis in rhesus monkeys after inhalational manganese exposure. | ||
Biochem Biophys Res Commun Myricetin ameliorates scopolamine-induced memory impairment in mice via inhibiting acetylcholinesterase and down-regulating brain iron. | ||
Brain Res Dysregulated iron metabolism in the choroid plexus in fragile X-associated tremor/ataxia syndrome. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sara (Verified Customer) (10-25-2020) | Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.
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