THOC1 Polyclonal antibody

THOC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10920-1-AP

Synonyms

HPR1, hTREX84, Nuclear matrix protein p84, P84, P84N5, THO complex 1, THO complex subunit 1, Tho1, THOC1



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10920-1-AP targets THOC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THOC1 fusion protein Ag1352
Full Name THO complex 1
Calculated Molecular Weight 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC010381
Gene Symbol THOC1
Gene ID (NCBI) 9984
RRIDAB_2202239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in promoted cancer cell apoptosis and reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy. This antibody recognizes the 84kd THOC1 protein.

Protocols

Product Specific Protocols
WB protocol for THOC1 antibody 10920-1-APDownload protocol
IHC protocol for THOC1 antibody 10920-1-APDownload protocol
IF protocol for THOC1 antibody 10920-1-APDownload protocol
IP protocol for THOC1 antibody 10920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Virol

SHFL inhibits enterovirus A71 infection by triggering degradation of viral 3Dpol protein via the ubiquitin-proteasome pathway

Authors - Chahui Tan
humanWB

Cancer Lett

TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.

Authors - Sai-Lan Liu
mouseWB

Elife

Post-transcriptional repression of circadian component CLOCK regulates cancer-stemness in murine breast cancer cells.

Authors - Takashi Ogino
humanWB,IF

Cancers (Basel)

Cytoplasmic Clusterin Suppresses Lung Cancer Metastasis by Inhibiting the ROCK1-ERK Axis.

Authors - Shaobo Huang
WB

Biochem Pharmacol

Modulation of cell physiology by bispecific nanobodies enabling changes in the intracellular localization of organelle proteins

Authors - Akito Tsuruta
humanWB

Cell Oncol (Dordr)

Bioactive peptide inhibits acute myeloid leukemia cell proliferation by downregulating ALKBH5-mediated m6A demethylation of EIF4EBP1 and MLST8 mRNA.

Authors - Le Zhang