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  • Featured Product
  • KD/KO Validated

THOC5 Polyclonal antibody

THOC5 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14862-1-AP

Synonyms

C22orf19, Fmip, fSAP79, hTREX90, KIAA0983, PK1.3, Placental protein 39.2, PP39.2, THO complex 5, THO complex subunit 5 homolog, THOC5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THOC5 Polyclonal antibody (14862-1-AP)

    WB analysis using 14862-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14862-1-AP (THOC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using THOC5 Polyclonal antibody (14862-1-AP)

    IP experiment of HEK-293 using 14862-1-AP

    IP result of anti-THOC5 (IP:14862-1-AP, 4ug; Detection:14862-1-AP 1:5000) with HEK-293 cells lysate 920 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using THOC5 Polyclonal antibody (14862-1-AP)

    IHC staining of human breast cancer using 14862-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THOC5 Polyclonal antibody (14862-1-AP)

    IHC staining of human lung cancer using 14862-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THOC5 Polyclonal antibody (14862-1-AP)

    IHC staining of human lung cancer using 14862-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using THOC5 Polyclonal antibody (14862-1-AP)

    IHC staining of human breast cancer using 14862-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using THOC5 Polyclonal antibody (14862-1-AP)

    IF Staining of HepG2 using 14862-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using THOC5 antibody (14862-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using THOC5 Polyclonal antibody (14862-1-AP)

    FC experiment of HEK-293 using 14862-1-AP

    1X10^6 HEK-293 cells were stained with 0.2ug THOC5 antibody (14862-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KOSee 1 publications below

Product Information

14862-1-AP targets THOC5 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THOC5 fusion protein Ag6664
Full Name THO complex 5
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC003615
Gene Symbol THOC5
Gene ID (NCBI) 8563
RRIDAB_2878088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THOC5, also named as C22orf19, or KIAA0983, is a 683 amino acid protein, which belongs to the THOC5 family. THOC5 is ubiquitously expressed in many tissues. THOC5 localizes in the nucleus and acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA.THOC5 plays a role in cytokine-stimulated monocytic development and affects granulocyte/macrophage differentiation.

Protocols

Product Specific Protocols
WB protocol for THOC5 antibody 14862-1-APDownload protocol
IHC protocol for THOC5 antibody 14862-1-APDownload protocol
IF protocol for THOC5 antibody 14862-1-APDownload protocol
IP protocol for THOC5 antibody 14862-1-APDownload protocol
FC protocol for THOC5 antibody 14862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Cell Stem Cell

The THO Complex Regulates Pluripotency Gene mRNA Export and Controls Embryonic Stem Cell Self-Renewal and Somatic Cell Reprogramming.

Authors - Wang Li L
  • KD Validated
View Article