TIMP1 Polyclonal antibody

TIMP1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10753-1-AP

Synonyms

CLGI, Collagenase inhibitor, EPA, HCI, Metalloproteinase inhibitor 1, TIMP, TIMP 1, TIMP1, TIMP-1



Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10753-1-AP targets TIMP1 in IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMP1 fusion protein Ag1146
Full Name TIMP metallopeptidase inhibitor 1
Calculated molecular weight 23 kDa
GenBank accession numberBC007097
Gene symbol TIMP1
Gene ID (NCBI) 7076
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tissue Inhibitor of Metalloproteinases-1 (TIMP1) is a naturally occurring protein in the human organism regulating the activity of matrix metalloproteinases (MMPs). TIMP1 encodes a 931 base-pair mRNA and a 207 amino acid protein. Overexpression of TIMP1 can lead to a substantially increase of genes involved in proliferation, apoptosis and signal transduction. In addition, TIMP1 was found to decrease tumor cell sensitivity to multiple anticancer drugs by activation of downstream pathways and exhibited anti-apoptotic activity. Specifically, the TIMP1 could degrade cyclinB1 and activate the NF-kβ signaling pathway to protect breast cancer cells against chemotherapy-induced cell death.

Protocols

Product Specific Protocols
IHC protocol for TIMP1 antibody 10753-1-APDownload protocol
IF protocol for TIMP1 antibody 10753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Biol Macromol

Anti-metastatic effects of antrodan, the Antrodia cinnamomea mycelia glycoprotein, in lung carcinoma cells.

Authors - Kuan-Ning Fa
mouseIHC

Exp Ther Med

PMID: 26622476

Authors - Mingxue Zhou
ratIHC

Exp Ther Med

Sijunzi decoction may decrease apoptosis via stabilization of the extracellular matrix following cerebral ischaemia-reperfusion in rats.

Authors - Ping Yang
mouseWB

Exp Lung Res

C-Kit/c-Kit ligand interaction of bone marrow endothelial progenitor cells is influenced in a cigarette smoke extract-induced emphysema model.

Authors - He Shengdong S

Mol Med Rep

Investigation of the expressions of MMPs and TIMPs between isogeneic and allogeneic rat aortic transplantation.

Authors - Si-Dong Wei
mouseWB

BMC Cardiovasc Disord

Effects of reduced β2-glycoprotein I on the expression of aortic matrix metalloproteinases and tissue inhibitor matrix metalloproteinases in diabetic mice.

Authors - Jun Xu