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  • KD/KO Validated

Transketolase Polyclonal antibody

Transketolase Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11039-1-AP

Synonyms

TK, TKT, TKT1, transketolase



Tested Applications

Positive WB detected inJurkat cells, rat liver tissue
Positive IHC detected inhuman lymphoma tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11039-1-AP targets Transketolase in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Transketolase fusion protein Ag1519
Full Name transketolase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC009970
Gene symbol TKT
Gene ID (NCBI) 7086
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer’s disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

Protocols

Product Specific Protocols
WB protocol for Transketolase antibody 11039-1-APDownload protocol
IHC protocol for Transketolase antibody 11039-1-APDownload protocol
IF protocol for Transketolase antibody 11039-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

J Exp Clin Cancer Res

Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.

Authors - Zhaoyu Qin
  • KD Validated
humanWB, IHC

Clin Exp Metastasis

Transketolase is upregulated in metastatic peritoneal implants and promotes ovarian cancer cell proliferation.

Authors - Carmela Ricciardelli
  • KD Validated
mouseWB

Proteomics

Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.

Authors - Xu Baogang J BJ
humanWB,IHC

Eur Respir J

Malignant pleural effusion cells show aberrant glucose metabolism gene expression.

Authors - Lin C-C CC
  • KD Validated
mouseWB

Proc Natl Acad Sci U S A

OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.

Authors - Ligong Chen