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- KD/KO Validated
Transketolase Polyclonal antibody
Transketolase Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11039-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, rat liver tissue |
| Positive IHC detected in | human lymphoma tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
11039-1-AP targets Transketolase in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Transketolase fusion protein Ag1519 |
| Full Name | transketolase |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC009970 |
| Gene symbol | TKT |
| Gene ID (NCBI) | 7086 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer’s disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Transketolase antibody 11039-1-AP | Download protocol |
| IHC protocol for Transketolase antibody 11039-1-AP | Download protocol |
| IF protocol for Transketolase antibody 11039-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.
| ||
Clin Exp Metastasis Transketolase is upregulated in metastatic peritoneal implants and promotes ovarian cancer cell proliferation.
| ||
Proteomics Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts. | ||
Eur Respir J Malignant pleural effusion cells show aberrant glucose metabolism gene expression.
| ||
Proc Natl Acad Sci U S A OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin. |
