|Positive WB detected in||Jurkat cells, rat liver tissue|
|Positive IHC detected in||human lymphoma tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||BxPC-3 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11039-1-AP targets Transketolase in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Transketolase fusion protein Ag1519|
|Calculated molecular weight||68 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC009970|
|Gene ID (NCBI)||7086|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer’s disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.
J Exp Clin Cancer Res
Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.
Clin Exp Metastasis
Transketolase is upregulated in metastatic peritoneal implants and promotes ovarian cancer cell proliferation.
Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
Eur Respir J
Malignant pleural effusion cells show aberrant glucose metabolism gene expression.
Proc Natl Acad Sci U S A
OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.