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TLR4 Antibody 65 Publications

Rabbit Polyclonal| Catalog number: 19811-1-AP

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Con: 35 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

TLR4 Antibody WB mouse liver tissue 19811-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP( TLR4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

TLR4 Antibody IHC human placenta tissue 19811-1-AP

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 19811-1-AP( TLR4 Antibody) at dilution of 1:200 (under 10x lens).

TLR4 Antibody IHC human placenta tissue 19811-1-AP

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 19811-1-AP( TLR4 Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat, hamster
Positive WB detected in:
mouse liver tissue, Daudi cells, rat liver tissue
Positive IHC detected in:
human placenta tissue, human liver cancer tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:100-1:400

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TLR4 fusion protein Ag13841
Full name:
toll-like receptor 4
Calculated molecular weight:
839aa,96 kDa
Observed molecular weight:
90-100 kDa
GenBank accession number:
BC117422
Gene ID (NCBI):
7099
Gene symbol
TLR4
Synonyms
ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4

Background

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described. The calculated molecular weights of the three TLR4 isoforms are 96, 91, and 73 kDa.


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