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  • KD/KO Validated

TMEM98 Polyclonal antibody

TMEM98 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14731-1-AP

Synonyms

Protein TADA1, TMEM98, transmembrane protein 98



Tested Applications

Positive WB detected inmouse E12.5 embryos
Positive IP detected inY79 cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14731-1-AP targets TMEM98 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM98 fusion protein Ag6492
Full Name transmembrane protein 98
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 21-25 kDa
GenBank Accession NumberBC000526
Gene Symbol TMEM98
Gene ID (NCBI) 26022
RRIDAB_11182589
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM98, also named as TADA1, composed of 226 amino acid residues, is a potential single-pass transmembrane protein. It is expression in both nucleus and cytoplasm. TMEM98 was found to be over-expressed in most of the hepatocellular carcinoma tissue. MEM98 is not only a prognostic marker for patients with hepatocellular carcinoma but also a novel molecular target associated with intrinsic and acquired chemoresistance of hepatocellular carcinoma (PMID: 24608572).

Protocols

Product Specific Protocols
IHC protocol for TMEM98 antibody 14731-1-APDownload protocol
IP protocol for TMEM98 antibody 14731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

PLoS Genet

The nanophthalmos protein TMEM98 inhibits MYRF self-cleavage and is required for eye size specification.

Authors - Sally H Cross
humanIF

PLoS Genet

Variants in myelin regulatory factor (MYRF) cause autosomal dominant and syndromic nanophthalmos in humans and retinal degeneration in mice.

Authors - Sarah J Garnai
humanWB

Aging (Albany NY)

The microRNA miR-29c-5p inhibits cell proliferation and migration by targeting TMEM98 in head and neck carcinoma.

Authors - Jingjia Li
mouseWB,IF

Invest Ophthalmol Vis Sci

Missense Mutations in the Human Nanophthalmos Gene TMEM98 Cause Retinal Defects in the Mouse.

Authors - Sally H Cross
  • KO Validated
humanWB,CoIP

PLoS One

TMEM98 is a negative regulator of FRAT mediated Wnt/ß-catenin signalling.

Authors - Tanne van der Wal
humanWB,IHC

Front Biosci (Landmark Ed)

Tumor-Suppressor Gene Transmembrane Protein 98 Promotes Proliferation and Inhibits Apoptosis in Ovarian Cancer

Authors - San-Gang Wu