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- KD/KO Validated
ASC/TMS1 Polyclonal antibody
ASC/TMS1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
207
Cat no : 10500-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HL-60 cells, A549 cells, Raji cells, U-937 cells, THP-1 cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human lung cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 182 publications below |
| IHC | See 21 publications below |
| IF | See 39 publications below |
| IP | See 5 publications below |
| ELISA | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
10500-1-AP targets ASC/TMS1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, pig, canine, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ASC/TMS1 fusion protein Ag0774 |
| Full Name | PYD and CARD domain containing |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC004470 |
| Gene Symbol | PYCARD |
| Gene ID (NCBI) | 29108 |
| RRID | AB_2174862 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
| IHC protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
| IF protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
| IP protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Research (Wash D C) Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis | ||
Transl Res IMM-H004 therapy for permanent focal ischemic cerebral injury via CKLF1/CCR4-mediated NLRP3 inflammasome activation. | ||
Cancer Res Silencing of TMS1/ASC promotes resistance to anoikis in breast epithelial cells.
| ||
Cancer Res Role of hMOF-dependent histone H4 lysine 16 acetylation in the maintenance of TMS1/ASC gene activity. | ||
Cell Mol Life Sci circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells | ||
Vaccines (Basel) Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages |
