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ASC/TMS1 Polyclonal antibody
ASC/TMS1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10500-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, A549 cells, Raji cells, U-937 cells |
Positive IP detected in | HL-60 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 52 publications below |
IHC | See 9 publications below |
IF | See 11 publications below |
Product Information
10500-1-AP targets ASC/TMS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | bovine, human, swine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ASC/TMS1 fusion protein Ag0774 |
Full Name | PYD and CARD domain containing |
Calculated molecular weight | 25 kDa |
Observed molecular weight | 22-25 kDa |
GenBank accession number | BC004470 |
Gene symbol | PYCARD |
Gene ID (NCBI) | 29108 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
IHC protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
IF protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
IP protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Chem Neuroanat Intrathecal transplantation of Wharton's jelly mesenchymal stem cells suppresses the NLRP1 inflammasome in the rat model of spinal cord injury. | ||
Int Immunopharmacol Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway. | ||
Theranostics p66Shc Contributes to Liver Fibrosis through the Regulation of Mitochondrial Reactive Oxygen Species. | ||
Front Physiol Weighted Gene Co-expression Network Analysis for RNA-Sequencing Data of the Varicose Veins Transcriptome. | ||
J Affect Disord Involvement of the microglial NLRP3 inflammasome in the anti-inflammatory effect of the antidepressant clomipramine. | ||