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ASC/TMS1 Polyclonal antibody

ASC/TMS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10500-1-AP

Synonyms

ASC, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1



Tested Applications

Positive WB detected inHL-60 cells, A549 cells, Raji cells, U-937 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10500-1-AP targets ASC/TMS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity bovine, human, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASC/TMS1 fusion protein Ag0774
Full Name PYD and CARD domain containing
Calculated molecular weight 25 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC004470
Gene symbol PYCARD
Gene ID (NCBI) 29108
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

Protocols

Product Specific Protocols
WB protocol for ASC/TMS1 antibody 10500-1-APDownload protocol
IHC protocol for ASC/TMS1 antibody 10500-1-APDownload protocol
IF protocol for ASC/TMS1 antibody 10500-1-APDownload protocol
IP protocol for ASC/TMS1 antibody 10500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Chem Neuroanat

Intrathecal transplantation of Wharton's jelly mesenchymal stem cells suppresses the NLRP1 inflammasome in the rat model of spinal cord injury.

Authors - Yousef Mohamadi
ratWB

Int Immunopharmacol

Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway.

Authors - Runxiao Lv
mouseWB

Theranostics

p66Shc Contributes to Liver Fibrosis through the Regulation of Mitochondrial Reactive Oxygen Species.

Authors - Yan Zhao
humanIHC

Front Physiol

Weighted Gene Co-expression Network Analysis for RNA-Sequencing Data of the Varicose Veins Transcriptome.

Authors - Jianbin Zhang
mouseWB

J Affect Disord

Involvement of the microglial NLRP3 inflammasome in the anti-inflammatory effect of the antidepressant clomipramine.

Authors - Wenqing Gong
mouseWB

Cell Death Dis

PMID: 31097691

Authors - Zhouqing Wang