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WB analysis using 10500-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 10500-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10500-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10500-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10500-1-AP (TMS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10500-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10500-1-AP (TMS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HL-60 using 10500-1-AP
IP result of anti-ASC/TMS1 (IP:10500-1-AP, 4ug; Detection:10500-1-AP 1:10000) with HL-60 cells lysate 2240 ug.
IHC staining of human lung cancer using 10500-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10500-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10500-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10500-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (TMS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 10500-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 10500-1-AP (TMS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ASC/TMS1 Polyclonal antibody

ASC/TMS1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10500-1-AP

Publications(300)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

PYCARD, hASC, Caspase recruitment domain-containing protein 5, CARD5, ASC

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (300)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HL-60 cells, A549 cells, Raji cells, U-937 cells, THP-1 cells
Positive IP detected in	HL-60 cells
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 265 publications below
IHC	See 24 publications below
IF	See 56 publications below
IP	See 7 publications below
ELISA	See 1 publications below
CoIP	See 4 publications below

Product Information

10500-1-AP targets ASC/TMS1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, pig, canine, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ASC/TMS1 fusion protein Ag0774 Predict reactive species
Full Name	PYD and CARD domain containing
Calculated Molecular Weight	25 kDa
Observed Molecular Weight	22-25 kDa
GenBank Accession Number	BC004470
Gene Symbol	ASC/TMS1
Gene ID (NCBI)	29108
RRID	AB_2174862
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9ULZ3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

Protocols

Product Specific Protocols
WB protocol for ASC/TMS1 antibody 10500-1-AP	Download protocol
IHC protocol for ASC/TMS1 antibody 10500-1-AP	Download protocol
IF protocol for ASC/TMS1 antibody 10500-1-AP	Download protocol
IP protocol for ASC/TMS1 antibody 10500-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Adv Sci (Weinh) A Heparan Sulfate Mimetic RAFT Copolymer Inhibits SARS-CoV-2 Infection and Ameliorates Viral-Induced Inflammation Authors - Jiaxin Ling View Article
human	WB	Nat Chem Biol E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells Authors - Siqi Shen View Article
human	WB	Research (Wash D C) Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis Authors - Lang Linghu View Article
	WB	Transl Res IMM-H004 therapy for permanent focal ischemic cerebral injury via CKLF1/CCR4-mediated NLRP3 inflammasome activation. Authors - Q D Ai View Article
	WB	Environ Pollut Disturbance of Mitochondrial Dynamics Led to Spermatogenesis Disorder in Mice Exposed to Polystyrene Micro- and Nanoplastics Authors - Moxuan Zhao View Article
human	WB,IHC	Cancer Res Silencing of TMS1/ASC promotes resistance to anoikis in breast epithelial cells. Authors - Parsons Melissa J MJ KD Validated View Article