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ASC/TMS1 Antibody 33 Publications

Rabbit Polyclonal| Catalog number: 10500-1-AP

Featured Product KD/KO validated

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

ASC/TMS1 Antibody WB HL-60 cells 10500-1-AP

HL-60 cells were subjected to SDS PAGE followed by western blot with 10500-1-AP(TMS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ASC/TMS1 Antibody IHC human lung cancer tissue 10500-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP( TMS1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ASC/TMS1 Antibody IHC human lung cancer tissue 10500-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP( TMS1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ASC/TMS1 Antibody IP HL-60 cells 10500-1-AP

IP result of anti-TMS1(IP:10500-1-AP, 4ug; Detection:10500-1-AP 1:1000) with HL-60 cells lysate 4000 ug

ASC/TMS1 Antibody IF MCF-7 cells 10500-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed MCF-7 cells using 10500-1-AP(TMS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, cow
Positive WB detected in:
HL-60 cells, A549 cells, Raji cells, U-937 cells
Positive IP detected in:
HL-60 cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ASC/TMS1 fusion protein Ag0774
Full name:
PYD and CARD domain containing
Calculated molecular weight:
25 kDa
Observed molecular weight:
22-25 kDa
GenBank accession number:
BC004470
Gene ID (NCBI):
29108
Gene symbol
PYCARD
Synonyms
ASC, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1

Background

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.


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