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TNRC4 Polyclonal antibody

TNRC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25353-1-AP

Synonyms

Bruno like protein 1, BRUNOL1, CAG repeat protein 4, CAGH4, CELF 3, CELF3, ERDA4, ETR 1, RNA binding protein BRUNOL 1, TNRC4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    WB analysis of mouse lung using 25353-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25353-1-AP (TNRC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    WB analysis of mouse spleen using 25353-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25353-1-AP (TNRC4 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human testis tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human testis using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human cerebellum using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human testis using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human cerebellum using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inmouse lung tissue, mouse spleen tissue
Positive IHC detected inhuman testis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25353-1-AP is TNRC4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNRC4 fusion protein Ag18601
Full Name trinucleotide repeat containing 4
Calculated Molecular Weight 464 aa, 51 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC052491
Gene Symbol TNRC4
Gene ID (NCBI) 11189
RRIDAB_2880041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNRC4, also named as BRUNOL1, CAGH4, ERDA4, or TNRC4, is a 465 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the CELF/BRUNOL family. TNRC4 localizes in the nucleus and is expressed in brain. TNRC4 as a RNA-binding protein is involved in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing.

Protocols

Product Specific Protocols
WB protocol for TNRC4 antibody 25353-1-APDownload protocol
IHC protocol for TNRC4 antibody 25353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols