p63 Antibody

Name: p63 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 12143-1-AP
Rating: 4.50 (2 reviews)

p63 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 12143-1-AP

Synonyms

AIS, B(p51A), B(p51B), CUSP, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, Tumor protein 63, tumor protein p63, Tumor protein p73 like

Validation Data Gallery

WB analysis of A431 cells using 12143-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 12143-1-AP(p63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours
IP experiment of A431 cells using 12143-1-AP
IP Result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:300) with A431 cells lysate 1200ug.
IHC staining of human breast hyperplasia tissue using 12143-1-AP
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 12143-1-AP( TP63 Antibody) at dilution of 1:200 (under 40x lens)
IHC staining of human tonsillitis tissue using 12143-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP( TP63 Antibody) at dilution of 1:200 (under 40x lens)
IF staining of A431 cells using 12143-1-AP
Immunofluorescent analysis of ( 10% Formaldehyde ) fixed A431 cells using 12143-1-AP(p63 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Cat No. 12143-1-AP

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Tested Applications

Positive WB detected in	A431 cells, Apoptosised HeLa cells, human heart tissue, mouse heart tissue, U-937 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human tonsillitis tissue, human skin cancer tissue, human breast hyperplasia tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	A431 cells

Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 3 publications below
IHC	See 3 publications below
IF	See 2 publications below

Product Information

12143-1-AP targets p63 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	p63 fusion protein Ag2791
Full Name	tumor protein p63
Calculated molecular weight	680aa,77 kDa
Observed molecular weight	63-68 kDa
GenBank accession number	BC039815
Gene symbol	TP63
Gene ID (NCBI)	8626
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

Protocols

Product Specific Protocols
WB protocol for p63 antibody 12143-1-AP	Download protocol
IHC protocol for p63 antibody 12143-1-AP	Download protocol
IF protocol for p63 antibody 12143-1-AP	Download protocol
IP protocol for p63 antibody 12143-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Res BCL-2 is a Therapeutic Target For Hypodiploid B-Lineage Acute Lymphoblastic Leukemia. Authors - Ernesto Diaz-Flores View Article
human	IF	Exp Eye Res Expansion and maintenance of primary corneal epithelial stem/progenitor cells by inhibition of TGFβ receptor I-mediated signaling. Authors - Lihua Hu View Article
rat	WB	Environ Toxicol Pharmacol All-trans-retinoid acid (ATRA) suppresses chondrogenesis of rat primary hind limb bud mesenchymal cells by downregulating p63 and cartilage-specific molecules. Authors - Yun-Guo Wang View Article
human	IHC	Prostate Molecular pathogenesis of human prostate basal cell hyperplasia. Authors - Gervaise Henry View Article
human	WB	Oxid Med Cell Longev NRF2 Is a Potential Modulator of Hyperresistance to Arsenic Toxicity in Stem-Like Keratinocytes. Authors - Xiafang Wu View Article
human	IHC	Cancers (Basel) Protective Effects of Dietary Grape on UVB-Mediated Cutaneous Damages and Skin Tumorigenesis in SKH-1 Mice. Authors - Charlotte A Mintie View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Tanusree (Verified Customer) (12-18-2019)	Product worked well in WB at 1:500 dilution Applications: Western Blot, Primary Antibody Dilution: 1:500 Cell Tissue Type: Mouse brain No image submitted with review
FH Kui (Verified Customer) (09-19-2019)	Best P63 Ab I can find so far. Applications: Immunofluorescence, Primary Antibody Dilution: 1:200 Cell Tissue Type: Mouse Esophagus p63 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse Esophagus (Cat no:12143-1-AP)