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- KD/KO Validated
TPT1 Polyclonal antibody
TPT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10824-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, mouse placenta tissue, MCF-7 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
10824-1-AP targets TPT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TPT1 fusion protein Ag1268 |
| Full Name | tumor protein, translationally-controlled 1 |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC003352 |
| Gene Symbol | TPT1 |
| Gene ID (NCBI) | 7178 |
| RRID | AB_2287617 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22-25 kDa TPT1 in lysates. An additional band around 50 kDa can also be observed occasionally, possibly representing the dimer form of TPT1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TPT1 antibody 10824-1-AP | Download protocol |
| IHC protocol for TPT1 antibody 10824-1-AP | Download protocol |
| IF protocol for TPT1 antibody 10824-1-AP | Download protocol |
| IP protocol for TPT1 antibody 10824-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Pharmacol Res Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect. | ||
Int J Biol Sci Ailanthus Altissima-derived Ailanthone enhances Gastric Cancer Cell Apoptosis by Inducing the Repression of Base Excision Repair by Downregulating p23 Expression. | ||
Chem Biol Interact AMG900 as novel inhibitor of the translationally controlled tumor protein. | ||
Invest New Drugs A novel ligand of the translationally controlled tumor protein (TCTP) identified by virtual drug screening for cancer differentiation therapy. | ||
BMC Cancer Identification of proteins involved in neural progenitor cell targeting of gliomas. | ||
Cancer Sci LncRNA TPT1-AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression.
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