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TRAF3 Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 18099-1-AP

Featured Product KD/KO validated

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Con: 63 μg/150 μl

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Tested Applications

Applications:
WB, IP, FC, ELISA
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Images See all

TRAF3 Antibody WB multi-cells 18099-1-AP

WB results of 18099-1-ap(anti TRAF3).

TRAF3 Antibody WB HEK-293 cells 18099-1-AP

WB result of TRAF3 antibody (18099-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAF3 transfected HEK-293 cells

TRAF3 Antibody WB HEK-293 cells 18099-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 18099-1-AP( TRAF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

TRAF3 Antibody IP Raji cells 18099-1-AP

IP result of anti-TRAF3(IP:18099-1-AP, 4ug; Detection:18099-1-AP 1:1000) with Raji cells lysate 3160 ug

TRAF3 Antibody FC Jurkat cells 18099-1-AP

1X10^6 Jurkat cells were stained with 0.2ug TRAF3 antibody (18099-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, sheep
Positive WB detected in:
HEK-293 cells, multi-cells , HeLa cells, COLO 320 cells, Raji cells
Positive IP detected in:
Raji cells
Positive FC detected in:
Jurkat cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TRAF3 fusion protein Ag12185
Full name:
TNF receptor-associated factor 3
Calculated molecular weight:
568aa,64 kDa
Observed molecular weight:
55-60 kDa
GenBank accession number:
BC075087
Gene ID (NCBI):
7187
Gene symbol
TRAF3
Synonyms
CAP 1, CAP1, CD40 binding protein, CD40bp, CRAF1, LAP1, LMP1 associated protein 1, TRAF3

Background

TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5. Several alternatively spliced transcript variants encoding distinct isoforms (64 kDa and 55 kDa) have been reported.


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