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- KD/KO Validated
TRAF3 Polyclonal antibody
TRAF3 Polyclonal Antibody for FC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IP, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 18099-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, multi-cells , HeLa cells, COLO 320 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 12 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 18099-1-AP is TRAF3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRAF3 fusion protein Ag12185 |
| Full Name | TNF receptor-associated factor 3 |
| Calculated molecular weight | 568 aa, 64 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC075087 |
| Gene symbol | TRAF3 |
| Gene ID (NCBI) | 7187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5. Several alternatively spliced transcript variants encoding distinct isoforms (64 kDa and 55 kDa) have been reported.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAF3 antibody 18099-1-AP | Download protocol |
| IP protocol for TRAF3 antibody 18099-1-AP | Download protocol |
| FC protocol for TRAF3 antibody 18099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Hypothermic oxygenated perfusion inhibits HECTD3-mediated TRAF3 polyubiquitination to alleviate DCD liver ischemia-reperfusion injury. | ||
Cell Rep Expression of miRNA-29 in Pancreatic β Cells Promotes Inflammation and Diabetes via TRAF3.
| ||
Mediators Inflamm Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells. | ||
J Biol Chem TNF receptor-associated factor 3 restrains B cell receptor signaling in normal and malignant B cells.
| ||
Front Microbiol TRAF3 Is Required for NF-κB Pathway Activation Mediated by HTLV Tax Proteins.
| ||
Front Pharmacol Protective Effects of Dioscin against Lipopolysaccharide-Induced Acute Lung Injury through Inhibition of Oxidative Stress and Inflammation. |
