Tested Applications
| Positive WB detected in | HeLa cells, COLO 320 cells, HEK-293 cells, multi-cells, Raji cells, Jurkat cells, NIH/3T3 cells |
| Positive IP detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 26 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
18099-1-AP targets TRAF3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRAF3 fusion protein Ag12185 Predict reactive species |
| Full Name | TNF receptor-associated factor 3 |
| Calculated Molecular Weight | 568 aa, 64 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC075087 |
| Gene Symbol | TRAF3 |
| Gene ID (NCBI) | 7187 |
| RRID | AB_10837364 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13114 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5. Several alternatively spliced transcript variants encoding distinct isoforms (64 kDa and 55 kDa) have been reported.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAF3 antibody 18099-1-AP | Download protocol |
| IP protocol for TRAF3 antibody 18099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion
| ||
Cell Death Differ The E3 ligase ASB3 downregulates antiviral innate immunity by targeting MAVS for ubiquitin-proteasomal degradation | ||
J Exp Med TRIM24 facilitates antiviral immunity through mediating K63-linked TRAF3 ubiquitination. | ||
Nucleic Acids Res The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response. | ||
EMBO J Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators.
| ||
EMBO Rep Estrogen receptor alpha signaling in dendritic cells modulates autoimmune disease phenotype in mice |
