|Positive WB detected in||A431 cells, HeLa cells, A431 cells, U2OS cells, HeLa cells, HepG2 cells, HEK-293 cells|
|Positive IHC detected in||mouse brain tissue, human heart tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66498-1-Ig targets TRAF6 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||TRAF6 fusion protein Ag24696|
|Full Name||TNF receptor-associated factor 6|
|Calculated molecular weight||522 aa, 60 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC031052|
|Gene ID (NCBI)||7189|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.
The Protective Effects of Levo-Tetrahydropalmatine on ConA-Induced Liver Injury Are via TRAF6/JNK Signaling.
J Biol Chem
The regulatory protein GADD34 inhibits TRAIL-induced apoptosis via TRAF6/ERK-dependent stabilization of myeloid cell leukemia 1 in liver cancer cells.
Arch Oral Biol
A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.
Oxid Med Cell Longev
Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury via Inhibiting TLR4/MyD88/NF-κB Signal Pathway In Vivo and In Vitro.
Oxid Med Cell Longev
Ferryl Hemoglobin Inhibits Osteoclastic Differentiation of Macrophages in Hemorrhaged Atherosclerotic Plaques.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.
Andrea (Verified Customer) (12-07-2018)
Antibody works well for western blot at a dilution of 1:8000. Did not work for immunoprecipitation.