- Featured Product
- KD/KO Validated
TRAF6 Monoclonal antibody
TRAF6 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1D1E1
Cat no : 66498-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, A431 cells, U2OS cells, HeLa cells, HepG2 cells, HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 19 publications below |
| IHC | See 1 publications below |
Product Information
66498-1-Ig targets TRAF6 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TRAF6 fusion protein Ag24696 |
| Full Name | TNF receptor-associated factor 6 |
| Calculated molecular weight | 522 aa, 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC031052 |
| Gene symbol | TRAF6 |
| Gene ID (NCBI) | 7189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAF6 antibody 66498-1-Ig | Download protocol |
| IHC protocol for TRAF6 antibody 66498-1-Ig | Download protocol |
| IF protocol for TRAF6 antibody 66498-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mediators Inflamm The Protective Effects of Levo-Tetrahydropalmatine on ConA-Induced Liver Injury Are via TRAF6/JNK Signaling. | ||
J Biol Chem The regulatory protein GADD34 inhibits TRAIL-induced apoptosis via TRAF6/ERK-dependent stabilization of myeloid cell leukemia 1 in liver cancer cells.
| ||
Arch Oral Biol A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis. | ||
Oxid Med Cell Longev Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury via Inhibiting TLR4/MyD88/NF-κB Signal Pathway In Vivo and In Vitro. | ||
Oxid Med Cell Longev Ferryl Hemoglobin Inhibits Osteoclastic Differentiation of Macrophages in Hemorrhaged Atherosclerotic Plaques. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
|
FH Andrea (Verified Customer) (12-07-2018) | Antibody works well for western blot at a dilution of 1:8000. Did not work for immunoprecipitation.
|
