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TRAF6 Antibody 4 Publications

Mouse Monoclonal| Catalog number: 66498-1-Ig |CloneNo.: 1D1E1

Featured Product KD/KO validated

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Con: 105 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

TRAF6 Antibody WB HeLa cells 66498-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66498-1-Ig (TRAF6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours

TRAF6 Antibody IHC mouse brain tissue 66498-1-Ig

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66498-1-Ig( TRAF6 antibody) at dilution of 1:600 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TRAF6 Antibody IHC mouse brain tissue 66498-1-Ig

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66498-1-Ig( TRAF6 antibody) at dilution of 1:600 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TRAF6 Antibody IF HeLa cells 66498-1-Ig

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 66498-1-Ig (TRAF6 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
Human, rat
Positive WB detected in:
HeLa cells
Positive IHC detected in:
mouse brain tissue, human heart tissue, human liver tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:12000
IHC : 1:300-1:1200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TRAF6 fusion protein Ag24696
Full name:
TNF receptor-associated factor 6
Calculated molecular weight:
522aa,60 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
BC031052
Gene ID (NCBI):
7189
Gene symbol
TRAF6
Synonyms
RING finger protein 85, RNF85, TRAF6

Background

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.


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