- Featured Product
- KD/KO Validated
TRAF6 Monoclonal antibody
TRAF6 Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1D1E1
Cat no : 66498-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HeLa cells, A431 cells, U2OS cells, HeLa cells, HepG2 cells, HEK-293 cells |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 21 publications below |
IHC | See 1 publications below |
Product Information
66498-1-Ig targets TRAF6 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TRAF6 fusion protein Ag24696 |
Full Name | TNF receptor-associated factor 6 |
Calculated molecular weight | 522 aa, 60 kDa |
Observed molecular weight | 60 kDa |
GenBank accession number | BC031052 |
Gene symbol | TRAF6 |
Gene ID (NCBI) | 7189 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TRAF6 antibody 66498-1-Ig | Download protocol |
IHC protocol for TRAF6 antibody 66498-1-Ig | Download protocol |
IF protocol for TRAF6 antibody 66498-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mediators Inflamm The Protective Effects of Levo-Tetrahydropalmatine on ConA-Induced Liver Injury Are via TRAF6/JNK Signaling. | ||
J Biol Chem The regulatory protein GADD34 inhibits TRAIL-induced apoptosis via TRAF6/ERK-dependent stabilization of myeloid cell leukemia 1 in liver cancer cells.
| ||
Arch Oral Biol A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis. | ||
Oxid Med Cell Longev Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury via Inhibiting TLR4/MyD88/NF-κB Signal Pathway In Vivo and In Vitro. | ||
Oxid Med Cell Longev Ferryl Hemoglobin Inhibits Osteoclastic Differentiation of Macrophages in Hemorrhaged Atherosclerotic Plaques. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
|
FH Andrea (Verified Customer) (12-07-2018) | Antibody works well for western blot at a dilution of 1:8000. Did not work for immunoprecipitation.
|