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TRAF6 Antibody 5 Publications

Mouse Monoclonal| Catalog number: 66498-1-Ig |CloneNo.: 1D1E1

Featured Product KD/KO validated

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Con: 105 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

TRAF6 Antibody WB HeLa cells 66498-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66498-1-Ig (TRAF6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours

TRAF6 Antibody IHC mouse brain tissue 66498-1-Ig

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66498-1-Ig( TRAF6 antibody) at dilution of 1:600 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TRAF6 Antibody IHC mouse brain tissue 66498-1-Ig

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66498-1-Ig( TRAF6 antibody) at dilution of 1:600 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TRAF6 Antibody IF HeLa cells 66498-1-Ig

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 66498-1-Ig (TRAF6 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
Human
Positive WB detected in:
HeLa cells
Positive IHC detected in:
mouse brain tissue, human heart tissue, human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:12000
IHC : 1:300-1:1200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TRAF6 fusion protein Ag24696
Full name:
TNF receptor-associated factor 6
Calculated molecular weight:
522aa,60 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
BC031052
Gene ID (NCBI):
7189
Gene symbol
TRAF6
Synonyms
RING finger protein 85, RNF85, TRAF6

Background

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

TRAF6 Mouse Monoclonal antibody

Andrea (Verified Customer), 12-07-2018
Applications
Western Blot,Immunoprecipitation,

Lot Number
Antibody works well for western blot at a dilution of 1:8000. Did not work for immunoprecipitation.

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