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  • KD/KO Validated

TRAF6 Monoclonal antibody

TRAF6 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D1E1

Cat no : 66498-1-Ig

Synonyms

RING finger protein 85, RNF85, TRAF6



Tested Applications

Positive WB detected inA431 cells, HeLa cells, A431 cells, U2OS cells, HeLa cells, HepG2 cells, HEK-293 cells
Positive IHC detected inmouse brain tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66498-1-Ig targets TRAF6 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAF6 fusion protein Ag24696
Full Name TNF receptor-associated factor 6
Calculated molecular weight 522 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC031052
Gene symbol TRAF6
Gene ID (NCBI) 7189
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

Protocols

Product Specific Protocols
WB protocol for TRAF6 antibody 66498-1-IgDownload protocol
IHC protocol for TRAF6 antibody 66498-1-IgDownload protocol
IF protocol for TRAF6 antibody 66498-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mediators Inflamm

The Protective Effects of Levo-Tetrahydropalmatine on ConA-Induced Liver Injury Are via TRAF6/JNK Signaling.

Authors - Qiang Yu
humanWB

J Biol Chem

The regulatory protein GADD34 inhibits TRAIL-induced apoptosis via TRAF6/ERK-dependent stabilization of myeloid cell leukemia 1 in liver cancer cells.

Authors - Peiying Song
  • KD Validated
humanWB

Arch Oral Biol

A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.

Authors - Shichen Sun
humanWB

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
mouseWB

Oxid Med Cell Longev

Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury via Inhibiting TLR4/MyD88/NF-κB Signal Pathway In Vivo and In Vitro.

Authors - Yichao Du
mouseWB

Oxid Med Cell Longev

Ferryl Hemoglobin Inhibits Osteoclastic Differentiation of Macrophages in Hemorrhaged Atherosclerotic Plaques.

Authors - Erzsébet Zavaczki

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Andrea (Verified Customer) (12-07-2018)

Antibody works well for western blot at a dilution of 1:8000. Did not work for immunoprecipitation.

  • Applications: Western Blot,Immunoprecipitation,