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  • Featured Product
  • KD/KO Validated

TRPC6 Polyclonal antibody

TRPC6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18236-1-AP

Synonyms

FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRPC6

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    WB analysis of mouse brain using 18236-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500.

  • Western Blot (WB) analysis of mouse lung tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    WB analysis of mouse lung using 18236-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    WB analysis of mouse lung using 18236-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TRPC6 Polyclonal antibody (18236-1-AP)

    WB analysis of COLO 320 using 18236-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    IP experiment of mouse lung using 18236-1-AP

    IP result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    IHC staining of human placenta using 18236-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    IHC staining of human placenta using 18236-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRPC6 Polyclonal antibody (18236-1-AP)

    IF Staining of HeLa using 18236-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRPC6 antibody (18236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A549 cells using TRPC6 Polyclonal antibody (18236-1-AP)

    FC experiment of A549 using 18236-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human TRPC6 (18236-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse brain, COLO 320 cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18236-1-AP targets TRPC6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC6 fusion protein Ag12976
Full Name transient receptor potential cation channel, subfamily C, member 6
Calculated Molecular Weight 931 aa, 106 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC093658
Gene Symbol TRPC6
Gene ID (NCBI) 7225
RRIDAB_10859822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

Protocols

Product Specific Protocols
WB protocol for TRPC6 antibody 18236-1-APDownload protocol
IHC protocol for TRPC6 antibody 18236-1-APDownload protocol
IF protocol for TRPC6 antibody 18236-1-APDownload protocol
IP protocol for TRPC6 antibody 18236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Cell

Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis.

Authors - Wei Rao
View Article
mouseWB,IF

Biomaterials

Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.

Authors - Meitian Wang
View Article
humanIF

Proc Natl Acad Sci U S A

TRPC6 channel translocation into phagosomal membrane augments phagosomal function.

Authors - Vladimir Riazanski
  • KO Validated
View Article
mouseWB

Phytomedicine

Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro

Authors - Zhongzhu Ai
View Article
mouseWB

Br J Pharmacol

Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.

Authors - Jiangnan Zhang
View Article
ratWB

Lab Invest

Amelioration of circadian disruption and calcium-handling protein defects by choline alleviates cardiac remodeling in abdominal aorta coarctation rats.

Authors - Xi He
View Article