Tested Applications
| Positive WB detected in | human placenta tissue, mouse kidney tissue, PC-3 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IF | See 5 publications below |
Product Information
13411-1-AP targets TRPV6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4060 Product name: Recombinant human TRPV6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-106 aa of BC034814 Sequence: MYVENHCSRPALLLQLWGRGSPAQARGWQGVRNSPVACSSPFRQEHCMSEHFKNRPACLGARSPPQGHKWGESPSQGTQAGAGKCRACGKRVSEGDRNGSGGGKWG Predict reactive species |
| Full Name | transient receptor potential cation channel, subfamily V, member 6 |
| Calculated Molecular Weight | 725 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa, 70 kDa |
| GenBank Accession Number | BC034814 |
| Gene Symbol | TRPV6 |
| Gene ID (NCBI) | 55503 |
| RRID | AB_2272390 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H1D0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRPV6, also named as CAT1 or ECaC2, is a member of the transient receptor potential (TRP) family of membrane proteins. Unlike most TRP channels, TRPV6 and its closest relative, TRPV5, are calcium-selective channels. TRPV6 is highly expressed in the proximal intestine, placenta and exocrine tissues (PMID: 12869611). It is probably involved in calcium absorption in various tissues, including calcium reabsorption in kidney. TRPV6 is overexpressed in some cancers and exhibits oncogenic potential.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRPV6 antibody 13411-1-AP | Download protocol |
| IP protocol for TRPV6 antibody 13411-1-AP | Download protocol |
| WB protocol for TRPV6 antibody 13411-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Mol Cell Cardiol TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes. | ||
Front Pharmacol Aqueous Extract of Mori Folium Exerts Bone Protective Effect Through Regulation of Calcium and Redox Homeostasis via PTH/VDR/CaBP and AGEs/RAGE/Nox4/NF-κB Signaling in Diabetic Rats. | ||
J Gastroenterol Hepatol Vitamin D receptor is overexpressed in the duodenum of patients with irritable bowel syndrome. | ||
Ann Transl Med Rosavin suppresses osteoclastogenesis in vivo and in vitro by blocking the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. |
