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Thrombospondin 1 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 18304-1-AP

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Con: 67 μg/150 μl

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Tested Applications
Applications:
ELISA, IF, IHC, IP, WB
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Images See all

18304-1-AP;HeLa cells

HeLa cells were subjected to SDS PAGE followed by western blot with 18304-1-AP(Thrombospondin 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

18304-1-AP;HeLa cells

IP Result of anti-Thrombospondin 1 (IP:18304-1-AP, 4ug; Detection:18304-1-AP 1:500) with HeLa cells lysate 2000ug.

18304-1-AP;human gliomas tissue

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 18304-1-AP( Thrombospondin 1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

18304-1-AP;human gliomas tissue

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 18304-1-AP( Thrombospondin 1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

18304-1-AP;MCF-7 cells

Immunofluorescent analysis of MCF-7 cells using 18304-1-AP(Thrombospondin 1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, rat, mouse
Positive WB detected in:
HeLa cells, A431 cells, A549 cells, C6 cells, HeLa cells, human heart tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human gliomas tissue
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
thrombospondin 1
Calculated molecular weight:
129 kDa
Observed molecular weight:
165-198 kDa
GenBank accession number:
BC028145
Gene ID (NCBI):
7057
Gene symbol
THBS1
Synonyms
THBS, THBS 1, THBS1, thrombospondin 1, TSP, TSP 1, TSP1
Background

TSP1, also named as THBS1, THBS and TSP, is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions, and which can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. TSP1 is a regulator of many biological processes including cell growth, adhesion, migration, platelet aggregation, and fibrin deposition and lysis. It interacts with a number of plasma proteins such as fibrinogen and plasminogen and co-polymerizes with fibrin in clot formation. TSP1 is a 420 kDa homotrimeric glycoprotein with disulfide-linked subunits of 180 kDa.


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