Thrombospondin 1 Polyclonal antibody
Thrombospondin 1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 18304-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | C6 cells, Human peripheral blood platelets tissue, A431 cells, HeLa cells, NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue, human placenta tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
18304-1-AP targets Thrombospondin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | thrombospondin 1 |
| Calculated molecular weight | 129 kDa |
| Observed molecular weight | 180 kDa |
| GenBank accession number | BC028145 |
| Gene symbol | THBS1 |
| Gene ID (NCBI) | 7057 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSP1, also named as THBS1, THBS and TSP, is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions, and which can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. TSP1 is a regulator of many biological processes including cell growth, adhesion, migration, platelet aggregation, and fibrin deposition and lysis. It interacts with a number of plasma proteins such as fibrinogen and plasminogen and co-polymerizes with fibrin in clot formation. Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Thrombospondin 1 antibody 18304-1-AP | Download protocol |
| IHC protocol for Thrombospondin 1 antibody 18304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction. | ||
Oral Surg Oral Med Oral Pathol Oral Radiol Upregulation of proangiogenic factors expression in the synovium of temporomandibular joint condylar hyperplasia. | ||
J Oral Pathol Med IL-1β-regulating angiogenic factors expression in perforated temporomandibular disk cells via NF-κB pathway. | ||
Biochimie Discovery of small extracellular vesicle proteins from human serum for liver cirrhosis and liver cancer. | ||
Front Neurosci Serum Exosomal Proteins F9 and TSP-1 as Potential Diagnostic Biomarkers for Newly Diagnosed Epilepsy. | ||
Oncotarget Novel circulating peptide biomarkers for esophageal squamous cell carcinoma revealed by a magnetic bead-based MALDI-TOFMS assay. |
