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WB analysis using 18304-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 18304-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 18304-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18304-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 18304-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human peripheral blood platelets using 18304-1-AP
Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Thrombospondin 1 Polyclonal antibody

Thrombospondin 1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18304-1-AP

Publications(53)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

THBS1, Thrombospondin-1, Glycoprotein G, THBS, THBS 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (53)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	C6 cells, A431 cells, HeLa cells, human peripheral blood platelets, HUVEC cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected in	human lung cancer tissue, human liver tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 43 publications below
IHC	See 16 publications below
IF	See 12 publications below

Product Information

18304-1-AP targets Thrombospondin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	thrombospondin 1
Calculated Molecular Weight	129 kDa
Observed Molecular Weight	180 kDa
GenBank Accession Number	BC028145
Gene Symbol	Thrombospondin 1
Gene ID (NCBI)	7057
RRID	AB_2201959
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07996
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

Protocols

Product Specific Protocols
WB protocol for Thrombospondin 1 antibody 18304-1-AP	Download protocol
IHC protocol for Thrombospondin 1 antibody 18304-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation. Authors - Yansen Xiao View Article
human	WB	Nat Commun The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets. Authors - Henry Nording View Article
mouse	IF	Nat Commun Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction. Authors - Juan Tang View Article
human	IHC	Cancer Lett Gastric cancer derived exosomal THBS1 enhanced Vγ9Vδ2 T-cell function through activating RIG-I-like receptor signaling pathway in a N6-methyladenosine methylation dependent manner Authors - Juntao Li View Article
rat	WB,IF	Transl Res Ntsr1 contributes to pulmonary hypertension by enhancing endoplasmic reticulum stress via JAK2-STAT3-Thbs1 signaling Authors - Zhi-Xing Wei View Article
rat	WB	J Med Chem Hypoxia Activated Nitric Oxide Donor Compounds for the Prevention and Treatment of Myocardial Hypoxia-Induced Injury Authors - Wanxiang Yang View Article