• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TTF2 Polyclonal antibody

TTF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13722-1-AP

Synonyms

F2, HuF2, Lodestar homolog, Transcription release factor 2, TTF2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A2780 cells using TTF2 Polyclonal antibody (13722-1-AP)

    WB analysis of A2780 using 13722-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TTF2 Polyclonal antibody (13722-1-AP)

    WB analysis using 13722-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using TTF2 Polyclonal antibody (13722-1-AP)

    WB analysis of A2780 using 13722-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A2780 cells using TTF2 Polyclonal antibody (13722-1-AP)

    IP experiment of A2780 using 13722-1-AP

    IP result of anti-TTF2 (IP:13722-1-AP, 4ug; Detection:13722-1-AP 1:500) with A2780 cells lysate 840ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TTF2 Polyclonal antibody (13722-1-AP)

    IHC staining of human ovary tumor using 13722-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13722-1-AP (TTF2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TTF2 Polyclonal antibody (13722-1-AP)

    IHC staining of human ovary tumor using 13722-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13722-1-AP (TTF2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TTF2 Polyclonal antibody (13722-1-AP)

    IF Staining of HeLa using 13722-1-AP

    Immunofluorescent analysis of Hela cells, using TTF2 antibody 13722-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inA2780 cells, HeLa cells, Jurkat cells
Positive IP detected inA2780 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13722-1-AP targets TTF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTF2 fusion protein Ag4671
Full Name transcription termination factor, RNA polymerase II
Calculated Molecular Weight 1162 aa, 129 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC030058
Gene Symbol TTF2
Gene ID (NCBI) 8458
RRIDAB_2262865
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription elongation is an key target for control of eukaryotic gene expression. After initiating from a promoter, the RNA polymerase II elongation complex is subjected to a block to elongation by the action of components of N-TEF (negative transcription elongation factor) [PMID: 9748214]. Transcription termination factor 2 (TTF2) is a DsDNA-dependent ATPase that acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. It also contribute to mitotic transcription repression, and involved in pre-mRNA splicing[PMID:15125840, 12927788].

Protocols

Product Specific Protocols
WB protocol for TTF2 antibody 13722-1-APDownload protocol
IHC protocol for TTF2 antibody 13722-1-APDownload protocol
IF protocol for TTF2 antibody 13722-1-APDownload protocol
IP protocol for TTF2 antibody 13722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

mRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription.

Authors - Brannan Kris K
View Article
ratWB

Sci Total Environ

PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism.

Authors - Mei Ha
View Article
humanIHC

Nat Commun

Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features

Authors - Weizhu Zhao
View Article