TXNL1 Polyclonal antibody
TXNL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15289-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, HL-60 cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human heart tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
15289-1-AP targets TXNL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TXNL1 fusion protein Ag7481 |
| Full Name | thioredoxin-like 1 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC001156 |
| Gene Symbol | TXNL1 |
| Gene ID (NCBI) | 9352 |
| RRID | AB_2288008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TXNL1(Thioredoxin-like protein 1) is also named as TRP32, TXL, TXNL and belongs to the thioredoxin family. It might be involved in the cellular response to sugar starvation stress. The predicted 289-amino acid, 32.3-kD protein has 2 distinct domains: an N-terminal domain, which is 43% identical to human TXN, and a C-terminal domain, which showed no homology to other proteins in the sequence databases(PMID:9473519).TXNL1 functions as an effector of oxidants or redox sensor that couples oxidative stress to endocytosis, by converting redox changes into a specific GDI:Rab5-mediated endocytic response(PMID:17987124).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TXNL1 antibody 15289-1-AP | Download protocol |
| IHC protocol for TXNL1 antibody 15289-1-AP | Download protocol |
| IF protocol for TXNL1 antibody 15289-1-AP | Download protocol |
| IP protocol for TXNL1 antibody 15289-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis TXNL1-XRCC1 pathway regulates cisplatin-induced cell death and contributes to resistance in human gastric cancer. | ||
Curr Cancer Drug Targets TXNL1 induces apoptosis in cisplatin resistant human gastric cancer cell lines. | ||
Vet Res Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1. |
