TXNL1 Polyclonal antibody

TXNL1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15289-1-AP

Synonyms

thioredoxin like 1, Thioredoxin like protein 1, TRP32, Txl, TXL 1, TXNL, TXNL1



Tested Applications

Positive WB detected inK-562 cells, HL-60 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman heart tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15289-1-AP targets TXNL1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNL1 fusion protein Ag7481
Full Name thioredoxin-like 1
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC001156
Gene symbol TXNL1
Gene ID (NCBI) 9352
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TXNL1(Thioredoxin-like protein 1) is also named as TRP32, TXL, TXNL and belongs to the thioredoxin family. It might be involved in the cellular response to sugar starvation stress. The predicted 289-amino acid, 32.3-kD protein has 2 distinct domains: an N-terminal domain, which is 43% identical to human TXN, and a C-terminal domain, which showed no homology to other proteins in the sequence databases(PMID:9473519).TXNL1 functions as an effector of oxidants or redox sensor that couples oxidative stress to endocytosis, by converting redox changes into a specific GDI:Rab5-mediated endocytic response(PMID:17987124).

Protocols

Product Specific Protocols
WB protocol for TXNL1 antibody 15289-1-APDownload protocol
IHC protocol for TXNL1 antibody 15289-1-APDownload protocol
IF protocol for TXNL1 antibody 15289-1-APDownload protocol
IP protocol for TXNL1 antibody 15289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Vet Res

Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1.

Authors - Caiying Wang
humanWB

Curr Cancer Drug Targets

TXNL1 induces apoptosis in cisplatin resistant human gastric cancer cell lines.

Authors - Pan Ni
humanWB,IHC

Cell Death Dis

TXNL1-XRCC1 pathway regulates cisplatin-induced cell death and contributes to resistance in human gastric cancer.

Authors - W Xu