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UBD Polyclonal antibody

UBD Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13003-2-AP

Synonyms

Diubiquitin, FAT10, GABBR1, UBD, UBD 3, ubiquitin D, Ubiquitin like protein FAT10



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

13003-2-AP targets UBD in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBD fusion protein Ag3680
Full Name ubiquitin D
Calculated molecular weight 165 aa, 18 kDa
GenBank accession numberBC012472
Gene symbol UBD
Gene ID (NCBI) 10537
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBD, also named as FAT10, contains two ubiquitin-like domains. It is a ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. UBD also has important roles in cell mitosis, chromosome instability, apoptosis and immune response. UBD mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). UBD regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. It may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T cell responses. UBD may be a marker for precancerous lesions and may promote cancer progression. This antibody is a rabbit polyclonal antibody raised against full length UBD of human origin.

Publications

SpeciesApplicationTitle

J Toxicol Sci

Disruption of spindle checkpoint function ahead of facilitation of cell proliferation by repeated administration of hepatocarcinogens in rats.

Authors - Masayuki Kimura
humanIHC

Exp Toxicol Pathol

Aberrant cell cycle regulation in rat liver cells induced by post-initiation treatment with hepatocarcinogens/hepatocarcinogenic tumor promoters.

Authors - Masayuki Kimura
ratIHC

J Appl Toxicol

Onset of hepatocarcinogen-specific cell proliferation and cell cycle aberration during the early stage of repeated hepatocarcinogen administration in rats.

Authors - Masayuki Kimura
mouseIF

Immunobiology

c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium.

Authors - Anuj Sehgal
ratIHC

Toxicol Lett

Ochratoxin A induces karyomegaly and cell cycle aberrations in renal tubular cells without relation to induction of oxidative stress responses in rats.

Authors - Taniai Eriko E
mouseIHC

DNA Res

Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.

Authors - Kobayashi Atsushi A