UBE2L3 Polyclonal antibody

UBE2L3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14415-1-AP

Synonyms

E2 F1, L UBC, UBCE7, UBCH7, UbcM4, UBE2L3, Ubiquitin carrier protein L3, Ubiquitin protein ligase L3



Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, K-562 cells, MCF-7 cells, mouse kidney tissue, mouse testis tissue, NIH/3T3 cells, rat kidney tissue
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

14415-1-AP targets UBE2L3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2L3 fusion protein Ag5758
Full Name ubiquitin-conjugating enzyme E2L 3
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC053368
Gene symbol UBE2L3
Gene ID (NCBI) 7332
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).

Protocols

Product Specific Protocols
WB protocol for UBE2L3 antibody 14415-1-APDownload protocol
IP protocol for UBE2L3 antibody 14415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Front Immunol

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Authors - Xu Ye
humanWB

J Cell Sci

A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.

Authors - Fabienne C Fiesel