UFM1 Polyclonal antibody

UFM1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15883-1-AP

Synonyms

BM 002, C13orf20, ubiquitin fold modifier 1, UFM1



Tested Applications

Positive WB detected inHT-29 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse lung tissue, L02 cells, U-251 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15883-1-AP targets UFM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UFM1 fusion protein Ag8667
Full Name ubiquitin-fold modifier 1
Calculated Molecular Weight 85 aa, 9 kDa
Observed Molecular Weight 9-12 kDa
GenBank Accession NumberBC005193
Gene Symbol UFM1
Gene ID (NCBI) 51569
RRIDAB_2878195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UFM1, also named as C13orf20, is a ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.

Protocols

Product Specific Protocols
WB protocol for UFM1 antibody 15883-1-APDownload protocol
IHC protocol for UFM1 antibody 15883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu