UFM1 Polyclonal antibody
UFM1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15883-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-29 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse lung tissue, L02 cells, U-251 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
15883-1-AP targets UFM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | UFM1 fusion protein Ag8667 |
Full Name | ubiquitin-fold modifier 1 |
Calculated Molecular Weight | 85 aa, 9 kDa |
Observed Molecular Weight | 9-12 kDa |
GenBank Accession Number | BC005193 |
Gene Symbol | UFM1 |
Gene ID (NCBI) | 51569 |
RRID | AB_2878195 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UFM1, also named as C13orf20, is a ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.
Protocols
Product Specific Protocols | |
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WB protocol for UFM1 antibody 15883-1-AP | Download protocol |
IHC protocol for UFM1 antibody 15883-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation |