UGT2B7 Polyclonal antibody
UGT2B7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16661-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat liver tissue, HEK-293 cells |
| Positive IP detected in | rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
16661-1-AP targets UGT2B7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UGT2B7 fusion protein Ag10121 |
| Full Name | UDP glucuronosyltransferase 2 family, polypeptide B7 |
| Calculated Molecular Weight | 529 aa, 61 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC030974 |
| Gene Symbol | UGT2B7 |
| Gene ID (NCBI) | 7364 |
| RRID | AB_2214249 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UGT2B7 antibody 16661-1-AP | Download protocol |
| IHC protocol for UGT2B7 antibody 16661-1-AP | Download protocol |
| IF protocol for UGT2B7 antibody 16661-1-AP | Download protocol |
| IP protocol for UGT2B7 antibody 16661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Divergent Expression and Metabolic Functions of Human Glucuronosyltransferases through Alternative Splicing. | ||
Br J Pharmacol UDP-Glucuronosyltransferase (UGT)-Mediated Attenuations of Cytochrome P450 3A4 Activity: UGT Isoform-Dependent Mechanism of Suppression. | ||
Sci Rep Inter-isoform Hetero-dimerization of Human UDP-Glucuronosyltransferases (UGTs) 1A1, 1A9, and 2B7 and Impacts on Glucuronidation Activity. | ||
Sci Rep Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues. | ||
Front Pharmacol Use of a Baculovirus-Mammalian Cell Expression-System for Expression of Drug-Metabolizing Enzymes: Optimization of Infection With a Focus on Cytochrome P450 3A4. |
