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UQCR Polyclonal antibody

UQCR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14793-1-AP

Synonyms

0710008D09Rik, Complex III subunit 10, Complex III subunit XI, Cytochrome b c1 complex subunit 10, QCR10, UQCR, UQCR11

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using UQCR Polyclonal antibody (14793-1-AP)

    WB analysis of MCF-7 using 14793-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14793-1-AP (UQCR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UQCR Polyclonal antibody (14793-1-AP)

    WB analysis using 14793-1-AP

    The lysate of total HeLa cells and mitochondria extracted from HeLa cells were subjected to SDS PAGE followed by western blot with 14793-1-AP (UQCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using UQCR Polyclonal antibody (14793-1-AP)

    IHC staining of human heart using 14793-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14793-1-AP (UQCR antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using UQCR Polyclonal antibody (14793-1-AP)

    IHC staining of human heart using 14793-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14793-1-AP (UQCR antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14793-1-AP targets UQCR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCR fusion protein Ag6491
Full Name ubiquinol-cytochrome c reductase, 6.4kDa subunit
Calculated Molecular Weight 6 kDa
GenBank Accession NumberBC000462
Gene Symbol UQCR
Gene ID (NCBI) 10975
RRIDAB_1557751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UQCR antibody 14793-1-APDownload protocol
IHC protocol for UQCR antibody 14793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Int J Mol Sci

Proteomic Study of Low-Birth-Weight Nephropathy in Rats.

Authors - Toshiyuki Imasawa
View Article