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USHBP1 Polyclonal antibody

USHBP1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17226-1-AP

Synonyms

AIE 75 binding protein, AIEBP, MCC 2, MCC2, USH1C binding protein 1, USHBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using USHBP1 Polyclonal antibody (17226-1-AP)

    WB analysis of A549 using 17226-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of COLO 320 cells using USHBP1 Polyclonal antibody (17226-1-AP)

    WB analysis of COLO 320 using 17226-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using USHBP1 Polyclonal antibody (17226-1-AP)

    WB analysis of mouse heart using 17226-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human heart tissue using USHBP1 Polyclonal antibody (17226-1-AP)

    IHC staining of human heart using 17226-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using USHBP1 Polyclonal antibody (17226-1-AP)

    IHC staining of human small intestine using 17226-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using USHBP1 Polyclonal antibody (17226-1-AP)

    IHC staining of human heart using 17226-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using USHBP1 Polyclonal antibody (17226-1-AP)

    IHC staining of human small intestine using 17226-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using USHBP1 Polyclonal antibody (17226-1-AP)

    IF Staining of A549 using 17226-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 17226-1-AP (USHBP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, COLO 320 cells, mouse heart tissue
Positive IHC detected inhuman heart tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17226-1-AP is USHBP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USHBP1 fusion protein Ag11064
Full Name Usher syndrome 1C binding protein 1
Calculated Molecular Weight 703 aa, 76 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC027910
Gene Symbol USHBP1
Gene ID (NCBI) 83878
RRIDAB_2878364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USHBP1, also named as Mutated in colon cancer protein 2 or AIE-75-binding protein, is a 703 amino acid protein, which belongs to the MCC family. Highest level of expression is in heart, and moderate to low expression in skeletal muscle, kidney, liver, small intestine, placenta and lung.

Protocols

Product Specific Protocols
WB protocol for USHBP1 antibody 17226-1-APDownload protocol
IHC protocol for USHBP1 antibody 17226-1-APDownload protocol
IF protocol for USHBP1 antibody 17226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols