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  • KD/KO Validated

USP15 Polyclonal antibody

USP15 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14354-1-AP

Synonyms

Deubiquitinating enzyme 15, KIAA0529, Ubiquitin thiolesterase 15, Unph 2, UNPH4, USP15



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14354-1-AP targets USP15 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP15 fusion protein Ag5703
Full Name ubiquitin specific peptidase 15
Calculated Molecular Weight 112 kDa
Observed Molecular Weight 112 kDa
GenBank Accession NumberBC020688
Gene Symbol USP15
Gene ID (NCBI) 9958
RRIDAB_2257148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for USP15 antibody 14354-1-APDownload protocol
IHC protocol for USP15 antibody 14354-1-APDownload protocol
IP protocol for USP15 antibody 14354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Immunol

USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses.

Authors - Qiang Zou
  • KO Validated
human,mouseWB

Nat Commun

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy

Authors - Xiangling Xiao
  • KD Validated
humanWB

Autophagy

USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.

Authors - Kaifeng Niu
  • KD Validated
mouseWB

Autophagy

LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a.

Authors - Haihai Liang
humanWB

Leukemia

The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia

Authors - Madeline Niederkorn
humanWB, IF

Sci Total Environ

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

Authors - Lei Zhang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Amy (Verified Customer) (11-08-2023)

Clean band for WB, used at a dilution lower than in the data sheet for U2OS cell lysates (1:500).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: U2OS