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- KD/KO Validated
USP8 Polyclonal antibody
USP8 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse and More (3)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 27791-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
27791-1-AP targets USP8 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | USP8 fusion protein Ag27143 |
| Full Name | ubiquitin specific peptidase 8 |
| Calculated Molecular Weight | 1118 aa, 128 kDa |
| Observed Molecular Weight | 128 kDa |
| GenBank Accession Number | BC110590 |
| Gene Symbol | USP8 |
| Gene ID (NCBI) | 9101 |
| RRID | AB_2880973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP8 antibody 27791-1-AP | Download protocol |
| IHC protocol for USP8 antibody 27791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer | ||
Sci Total Environ USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway. | ||
Aging Dis Exogenous H2S Promoted USP8 Sulfhydration to Regulate Mitophagy in the Hearts of db/db Mice. | ||
Cancer Manag Res Down-regulation of USP8 Inhibits Cholangiocarcinoma Cell Proliferation and Invasion.
| ||
Front Oncol Knockdown of USP8 inhibits prostate cancer cell growth, proliferation, and metastasis and promotes docetaxel’s activity by suppressing the NF-kB signaling pathway
| ||
Cancers (Basel) The Expression of Cell Cycle-Related Genes in USP8-Mutated Corticotroph Neuroendocrine Pituitary Tumors and Their Possible Role in Cell Cycle-Targeting Treatment |
