|Positive WB detected in||HEK-293 cells, HeLa cells, L02 cells, mouse skeletal muscle tissue, mouse testis tissue|
|Positive IHC detected in||human colon cancer tissue, human brain tissue, human kidney tissue, human lung tissue, human medulloblastoma tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12195-1-AP targets VPS33B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPS33B fusion protein Ag2833|
|Full Name||vacuolar protein sorting 33 homolog B (yeast)|
|Calculated molecular weight||617 aa, 71 kDa|
|Observed molecular weight||71 kDa|
|GenBank accession number||BC016445|
|Gene ID (NCBI)||26276|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).
Int J Cancer
VPS33B negatively modulated by nicotine functions as a tumor suppressor in colorectal cancer.
Clin Kidney J
Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome.
Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7.
Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors.
J Biol Chem
Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.
J Cell Sci
Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
RICHA (Verified Customer) (11-03-2020)
Excellent antibody for wester and IF.