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VPS33B Antibody

VPS33B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12195-1-AP

Synonyms

hVPS33B, VPS33B



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, L02 cells, mouse skeletal muscle tissue, mouse testis tissue
Positive IHC detected inhuman colon cancer tissue, human brain tissue, human kidney tissue, human lung tissue, human medulloblastoma tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12195-1-AP targets VPS33B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS33B fusion protein Ag2833
Full Name vacuolar protein sorting 33 homolog B (yeast)
Calculated molecular weight 617aa,71 kDa
Observed molecular weight 71 kDa
GenBank accession number BC016445
Gene symbol VPS33B
Gene ID (NCBI) 26276
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Protocols

Product Specific Protocols
WB protocol for VPS33B antibody 12195-1-APDownload protocol
IHC protocol for VPS33B antibody 12195-1-APDownload protocol
IF protocol for VPS33B antibody 12195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Cancer

VPS33B negatively modulated by nicotine functions as a tumor suppressor in colorectal cancer.

Authors - Yiyu Chen
humanWB

Clin Kidney J

Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome.

Authors - Amelia Holme
humanWB

Virus Genes

Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7.

Authors - Ye Li
zebrafishIF

Sci Rep

Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors.

Authors - Yu Liu
humanWB, IF

J Biol Chem

Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.

Authors - Rik van der Kant
humanIF

J Cell Sci

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Authors - van der Kant Rik R