VTI1A Polyclonal antibody

VTI1A Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12354-1-AP

Synonyms

MVti1, Vti1 rp2, VTI1A



Tested Applications

Positive WB detected inhuman brain tissue, human heart tissue, human kidney tissue, human lung tissue, MCF-7 cells, mouse brain tissue
Positive IP detected inMCF-7 cells
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12354-1-AP targets VTI1A in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VTI1A fusion protein Ag3018
Full Name vesicle transport through interaction with t-SNAREs homolog 1A (yeast)
Calculated Molecular Weight 217 aa, 25 kDa
Observed Molecular Weight 25-29 kDa
GenBank Accession NumberBC017052
Gene Symbol VTI1A
Gene ID (NCBI) 143187
RRIDAB_2216150
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). VTI1A is involved in transport between the endosome and the trans-Golgi network. The SNARE complex of STX10, STX16, VTI1A, and VAMP3 is required for MPR (mannose 6-phosphate receptors) transport from endosomes to the Golgi after delivering lysosomal enzymes to the endocytic pathway (PMID: 18195106). Transport from early/recycling endosomes to the TGN is mediated by the SNARE complex of STX6, STX16, VTI1A and VAMP4 (PMID: 11839770; 21807881).

Protocols

Product Specific Protocols
WB protocol for VTI1A antibody 12354-1-APDownload protocol
IF protocol for VTI1A antibody 12354-1-APDownload protocol
IP protocol for VTI1A antibody 12354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Cell Biol

The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport.

Authors - Laufman Orly O
humanWB,IF

J Cell Sci

The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.

Authors - Laufman Orly O
humanWB

Oncotarget

Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.

Authors - Yi Huo
humanWB,IF

Traffic

Deficiency of the Cog8 subunit in normal and CDG-derived cells impairs the assembly of the COG and Golgi SNARE complexes.

Authors - Laufman Orly O
humanWB

Biochem J

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.

Authors - Nazma Malik
mouseWB

Proc Natl Acad Sci U S A

Nanoelectrochemistry reveals how soluble Aβ42 oligomers alter vesicular storage and release of glutamate

Authors - Xiao-Ke Yang