• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CRM1 Polyclonal antibody

CRM1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 12915-1-AP

Synonyms

CRM1, Exp1, Exportin 1, XPO1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HT-1080 cells using CRM1 Polyclonal antibody (12915-1-AP)

    WB analysis of HT-1080 using 12915-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12915-1-AP (CRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CRM1 Polyclonal antibody (12915-1-AP)

    IHC staining of human kidney using 12915-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12915-1-AP (CRM1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CRM1 Polyclonal antibody (12915-1-AP)

    IHC staining of human kidney using 12915-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12915-1-AP (CRM1 antibody) at dilution of 1:50 (under 40x lens).

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 12915-1-AP is CRM1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRM1 fusion protein Ag3488
Full Name exportin 1 (CRM1 homolog, yeast)
Calculated Molecular Weight 1071 aa, 123 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC032847
Gene Symbol XPO1
Gene ID (NCBI) 7514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

Publications

SpeciesApplicationTitle
humanWB

Toxicology

Tetrachlorobenzoquinone induces Nrf2 activation via rapid Bach1 nuclear export/ubiquitination and JNK-P62 signaling.

Authors - Chuanyang Su
View Article