Tested Applications
| Positive WB detected in | HEK-293 cells, C6 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, K-562 cells, HT-1080 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27917-1-AP targets CRM1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27207 Product name: Recombinant human XPO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 301-540 aa of BC032847 Sequence: NTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHDQLIEKRLNLRETLMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDVPPRRQLYLPMLFKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTERIMTEKLHNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAII Predict reactive species |
| Full Name | exportin 1 (CRM1 homolog, yeast) |
| Calculated Molecular Weight | 1071 aa, 123 kDa |
| Observed Molecular Weight | 123 kDa |
| GenBank Accession Number | BC032847 |
| Gene Symbol | CRM1 |
| Gene ID (NCBI) | 7514 |
| RRID | AB_2881009 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14980 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CRM1 antibody 27917-1-AP | Download protocol |
| IHC protocol for CRM1 antibody 27917-1-AP | Download protocol |
| WB protocol for CRM1 antibody 27917-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CRM1 Polyclonal antibody (Cat# 27917-1-AP) has 10 citations published in journals including Cancer Research, Molecular Cell, Frontiers in Immunology, Cells, FASEB Journal, Journal of Biological Chemistry, Journal of Immunology, Journal of Cell Communication and Signaling, and Journal of Cancer Research and Clinical Oncology. Research fields span oncology (esophageal, glioblastoma, hepatocellular, penile cancers), tumor immunology, RNA metabolism (m6A/circRNA), stroke pathology, autophagy, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Cancer Res Long Noncoding RNA VESTAR Regulates Lymphangiogenesis and Lymph Node Metastasis of Esophageal Squamous Cell Carcinoma by Enhancing VEGFC mRNA Stability | ||
Mol Cell A GAPDH serotonylation system couples CD8+ T cell glycolytic metabolism to antitumor immunity | ||
Mol Cell EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma | ||
Front Immunol Integrative multi-omics analysis reveals a novel subtype of hepatocellular carcinoma with biological and clinical relevance | ||
Cells FSH-Induced Nuclear Exclusion of FOXO1 Mediated by PI3K/Akt Signaling Pathway in Granulosa Cells Is Associated with Follicle Selection and Growth of the Hen Ovary. | ||
FASEB J CRM-1 Aggravates Stroke Injury by Promoting BANF1-Mediated Unfolded Protein Response Through Inducing Nuclear Export of ALKBH5. |
Reviews
What customers say
Both reviewers gave this product a perfect 5-star rating. One user reported excellent band quality when using it at a 1:5000 dilution for Western Blot on HEK293T cells. Another user noted strong, clear bands when testing mouse heart lysates by Western Blot. Overall, customers are satisfied with the antibody's performance, highlighting its ability to produce sharp and reliable bands across different sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xingyuan (Verified Customer) (07-17-2023) | The band quality was pretty good
 |
Wei (Verified Customer) (03-12-2020) | strong, clear band for mouse heart lysates
|
