• Featured Product
  • KD/KO Validated

YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, CLIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 24744-1-AP

Synonyms

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, MOLT-4 cells, NIH/3T3 cells, C6 cells, Raji cells
Positive IP detected inHeLa cells
Positive IF detected inmouse brain tissue, U2OS cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24744-1-AP targets YTHDF2 in WB, RIP, IP, IHC, IF, FC, CoIP, CLIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF2 fusion protein Ag5922
Full Name YTH domain family, member 2
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC002559
Gene Symbol YTHDF2
Gene ID (NCBI) 51441
RRIDAB_2687435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.

Protocols

Product Specific Protocols
WB protocol for YTHDF2 antibody 24744-1-APDownload protocol
IF protocol for YTHDF2 antibody 24744-1-APDownload protocol
IP protocol for YTHDF2 antibody 24744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

m6A enhances the phase separation potential of mRNA.

Authors - Ryan J Ries
mouseRIP

Nature

m6A RNA methylation regulates the fate of endogenous retroviruses.

Authors - Tomasz Chelmicki
  • KO Validated
mouseWB

Nature

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Authors - Hailing Shi
human, mouseWB, IF

Nature

Dynamic m(6)A mRNA methylation directs translational control of heat shock response.

Authors - Jun Zhou
humanWB

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
human,mouseFC

Nat Med

Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy.

Authors - Irene Ganan-Gomez

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jinyun (Verified Customer) (08-26-2022)

I did the western blot, and the antibody worked quite well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
FH

Robert (Verified Customer) (01-24-2019)

1. scrambled siRNA2. YTHDF2 siRNA

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
YTHDF2 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:24744-1-AP)