ZCCHC11 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 18980-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 30 μg/150 μl

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Species specificity:
human, mouse

Positive WB detected in:
MCF7 cells, HEK-293T cells, HeLa cells, human brain tissue

Positive IP detected in:
MCF-7 cells

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
zinc finger, CCHC domain containing 11

Calculated molecular weight:
185 kDa

Observed molecular weight:
185 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ZCCHC11

Synonyms
KIAA0191, PAPD3, Terminal uridylyltransferase 4, TUT4, TUTase 4, ZCCHC11
Background

ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can’t bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.


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