Tested Applications
| Positive WB detected in | MCF7 cells, HEK-293T cells, HeLa cells, human brain tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 11 publications below |
| WB | See 24 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
18980-1-AP targets ZCCHC11 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | zinc finger, CCHC domain containing 11 |
| Calculated Molecular Weight | 185 kDa |
| Observed Molecular Weight | 185 kDa |
| GenBank Accession Number | NM_015269 |
| Gene Symbol | ZCCHC11 |
| Gene ID (NCBI) | 23318 |
| RRID | AB_10598327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TAX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZCCHC11 antibody 18980-1-AP | Download protocol |
| IP protocol for ZCCHC11 antibody 18980-1-AP | Download protocol |
| WB protocol for ZCCHC11 antibody 18980-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature mRNA 3' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome.
| ||
Nature A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.
| ||
Cell Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.
| ||
Mol Cell 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.
| ||
