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  • Featured Product
  • KD/KO Validated

HIP14; ZDHHC17 Polyclonal antibody

HIP14; ZDHHC17 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15465-1-AP

Synonyms

DHHC 17, HIP 14, HIP 3, HIP14, HIP14; ZDHHC17, HIP3, HSPC294, Huntingtin yeast partner H, HYPH, KIAA0946, Palmitoyltransferase ZDHHC17, ZDHHC17

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using HIP14; ZDHHC17 Polyclonal antibody (15465-1-AP)

    WB analysis of Raji using 15465-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using HIP14; ZDHHC17 Polyclonal antibody (15465-1-AP)

    WB analysis of human kidney using 15465-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using HIP14; ZDHHC17 Polyclonal antibody (15465-1-AP)

    IHC staining of human testis using 15465-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HIP14; ZDHHC17 Polyclonal antibody (15465-1-AP)

    IHC staining of human testis using 15465-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inRaji cells, human kidney tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15465-1-AP targets HIP14; ZDHHC17 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIP14; ZDHHC17 fusion protein Ag7763
Full Name zinc finger, DHHC-type containing 17
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC050324
Gene Symbol ZDHHC17
Gene ID (NCBI) 23390
RRIDAB_2217690
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.

Protocols

Product Specific Protocols
WB protocol for HIP14; ZDHHC17 antibody 15465-1-APDownload protocol
IHC protocol for HIP14; ZDHHC17 antibody 15465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF, WB

J Cell Biol

Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.

Authors - Thaddaeus Mutugi Nthiga
View Article
humanWB

mBio

Identification of ZDHHC17 as a Potential Drug Target for Swine Acute Diarrhea Syndrome Coronavirus Infection

Authors - Yun Luo
  • KO Validated
View Article
WB

J Cell Sci

Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus

Authors - Danya Abazari
View Article
mouseIHC

Mol Cell Endocrinol

ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells

Authors - Shanmeizi Zhao
View Article