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SARA Polyclonal antibody

SARA Polyclonal Antibody for FC, IF, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14821-1-AP

Synonyms

hSARA, Madh interacting protein, MADHIP, Novel serine protease, NSP, Receptor activation anchor, SARA, SMADIP, ZFYVE9



Tested Applications

Positive IP detected inmouse brain tissue
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14821-1-AP targets SARA in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARA fusion protein Ag6592
Full Name zinc finger, FYVE domain containing 9
Calculated molecular weight 1425 aa, 156 kDa
GenBank accession numberBC032680
Gene symbol ZFYVE9
Gene ID (NCBI) 9372
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZFYVE9, also known as SARA (Smad anchor for receptor activation), is a double zinc finger (FYVE domain) protein that interacts directly with SMAD2 and SMAD3, and is involved in Alzheimer's disease. SARA functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. SARA plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. SARA is possibly associated with TGF-beta receptor internalization.

Protocols

Product Specific Protocols
WB protocol for SARA antibody 14821-1-APDownload protocol
IF protocol for SARA antibody 14821-1-APDownload protocol
IP protocol for SARA antibody 14821-1-APDownload protocol
FC protocol for SARA antibody 14821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Stem Cell Reports

Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool.

Authors - Xuanmao Jiao
humanWB, IF

J Biol Chem

Phosphatidylinositol 3-kinase and Rab5 GTPase inversely regulate the Smad anchor for receptor activation (SARA) protein independently of transforming growth factor-β1.

Authors - Runyan Constance E CE
humanWB

Oncotarget

TGFBR-IDH1-Cav1 axis promotes TGF-β signalling in cancer-associated fibroblast.

Authors - Xiaodan Hou
mouseIF

Mol Psychiatry

WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling.

Authors - Min Wang
  • KD Validated