|Positive IP detected in||mouse brain tissue|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14821-1-AP targets SARA in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SARA fusion protein Ag6592|
|Full Name||zinc finger, FYVE domain containing 9|
|Calculated molecular weight||1425 aa, 156 kDa|
|GenBank accession number||BC032680|
|Gene ID (NCBI)||9372|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ZFYVE9, also known as SARA (Smad anchor for receptor activation), is a double zinc finger (FYVE domain) protein that interacts directly with SMAD2 and SMAD3, and is involved in Alzheimer's disease. SARA functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. SARA plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. SARA is possibly associated with TGF-beta receptor internalization.
|Product Specific Protocols|
|WB protocol for SARA antibody 14821-1-AP||Download protocol|
|IF protocol for SARA antibody 14821-1-AP||Download protocol|
|IP protocol for SARA antibody 14821-1-AP||Download protocol|
|FC protocol for SARA antibody 14821-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Stem Cell Reports
Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool.
J Biol Chem
Phosphatidylinositol 3-kinase and Rab5 GTPase inversely regulate the Smad anchor for receptor activation (SARA) protein independently of transforming growth factor-β1.
TGFBR-IDH1-Cav1 axis promotes TGF-β signalling in cancer-associated fibroblast.
WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling.