- Featured Product
- KD/KO Validated
Beta Catenin Polyclonal antibody
Beta Catenin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 17565-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | C6 cells, mouse brain tissue, HeLa cells, NIH/3T3 cells, A431 cells, rat brain tissue, HEK-293 cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | T-47D cells, A431 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 45 publications below |
| IHC | See 9 publications below |
| IF | See 20 publications below |
| IP | See 1 publications below |
Product Information
17565-1-AP targets Beta Catenin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Beta Catenin fusion protein Ag11763 |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Calculated molecular weight | 781 aa, 86 kDa |
| Observed molecular weight | 92 kDa |
| GenBank accession number | BC058926 |
| Gene symbol | CTNNB1 |
| Gene ID (NCBI) | 1499 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
| IHC protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
| IF protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Physiol WISP1 silencing confers protection against epithelial-mesenchymal transition of renal tubular epithelial cells in rats via inactivation of the wnt/β-catenin signaling pathway in uremia. | ||
J Control Release Alginate/Chitosan microparticles for gastric passage and intestinal release of therapeutic protein nanoparticles. | ||
Biochem Cell Biol The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway. | ||
Cancer Sci LncRNA MIR22HG inhibits growth, migration and invasion through regulating the miR-10a-5p/NCOR2 axis in hepatocellular carcinoma cells. | ||
In Vitro Cell Dev Biol Anim miR-145-5p suppresses osteogenic differentiation of adipose-derived stem cells by targeting semaphorin 3A. | ||
Eur Rev Med Pharmacol Sci MiR-202-3p functions as a tumor suppressor and reduces cell migration and invasion in papillary thyroid carcinoma. |
