Name: beta-Catenin Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee

beta-Catenin Antibody WB C6 cells 17565-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours

beta-Catenin Antibody IF A431 cells 17565-1-AP

Immunofluorescent analysis of A431 cells using 17565-1-AP(beta-Catenin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

beta-Catenin Antibody IHC human breast cancer tissue 17565-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17565-1-AP( beta-Catenin antibody at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17565-1-AP( beta-Catenin antibody at dilution of 1:50 (under 40x lens)

Species specificity:

human, mouse, rat, zebrafish

Positive WB detected in:

C6 cells, mouse brain tissue, HeLa cells, NIH/3T3 cells, A431 cells, rat brain tissue, HEK-293 cells, MCF-7 cells, mouse liver tissue, rat liver tissue

Positive IHC detected in:

human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

A431 cells, MCF-7 cells

Recommended dilution:

WB : 1:2000-1:12000

IHC : 1:50-1:500

IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

beta-Catenin fusion protein Ag11763

Full name:

catenin (cadherin-associated protein), beta 1, 88kDa

Calculated molecular weight:

781aa,86 kDa

Observed molecular weight:

92 kDa

GenBank accession number:

BC058926

Gene ID (NCBI):

1499

Gene symbol

CTNNB1

Synonyms

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin

Background

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Showing 1- [6] of [17] Publications

Author:		Jiaye Wang
Pubmed ID:		29626521
Journal:		Toxicol Lett
Title:		Wnt/β-catenin modulates chronic tobacco smoke exposure-induced acquisition of pulmonary cancer stem cell properties and diallyl trisulfide intervention.
Application:		WB
KD validated
Species:		human

Author:		Tao Li
Pubmed ID:		29725434
Journal:		Oncol Lett
Title:		Inhibitory effects of aesculetin on the proliferation of colon cancer cells by the Wnt/β-catenin signaling pathway.
Application:		WB

Species:		human

Author:		Xue Shi
Pubmed ID:		29729281
Journal:		Toxicol Appl Pharmacol
Title:		Salvianolic acid A alleviates chronic ethanol-induced liver injury via promotion of β-catenin nuclear accumulation by restoring SIRT1 in rats.
Application:		WB,IP,IF

Species:		mouse

Author:		Yue Zhang
Pubmed ID:		29970879
Journal:		Cell Death Dis
Title:		Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.
Application:		WB,IF

Species:

Author:		Ruosi Yao
Pubmed ID:		30210697
Journal:		Am J Transl Res
Title:		Lithium chloride inhibits cell survival, overcomes drug resistance, and triggers apoptosis in multiple myeloma via activation of the Wnt/β-catenin pathway.
Application:		WB

Species:		human

Author:		Feng Zhang
Pubmed ID:		30358003
Journal:		Cell Biol Int
Title:		Klotho gene-modified BMSCs showed elevated antifibrotic effects by inhibiting the Wnt/β-catenin pathway in kidneys after acute injury.
Application:		IHC

Species:		mouse

WB Figures

beta-Catenin Antibody WB A431 cells 17565-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

Various lysates were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours

beta-Catenin Antibody WB HEK-293 cells 17565-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

beta-Catenin Antibody WB HeLa cells 17565-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

beta-Catenin Antibody WB MCF-7 cells 17565-1-AP

MCF-7 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

beta-Catenin Antibody WB mouse brain tissue 17565-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

beta-Catenin Antibody WB mouse liver tissue 17565-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

beta-Catenin Antibody WB NIH/3T3 cells 17565-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

beta-Catenin Antibody WB rat brain tissue 17565-1-AP

rat brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17565-1-AP( beta-Catenin antibody at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17565-1-AP( beta-Catenin antibody at dilution of 1:50 (under 40x lens)

IF Figures

Immunofluorescent analysis of A431 cells using 17565-1-AP(beta-Catenin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

beta-Catenin Antibody IF MCF-7 cells 17565-1-AP

Immunofluorescent analysis of MCF-7 cells using 17565-1-AP(beta-Catenin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

beta-Catenin Antibody 17 Publications

Rabbit Polyclonal| Catalog number: 17565-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [6] of [17] Publications

WB Figures

IHC Figures

IF Figures

beta-Catenin Antibody 17 Publications

Rabbit Polyclonal| Catalog number: 17565-1-AP

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Special offer: Add control/tag antibody for $99

Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background