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Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17565-1-AP

Synonyms

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin



Tested Applications

Positive WB detected inC6 cells, mouse brain tissue, HeLa cells, NIH/3T3 cells, A431 cells, rat brain tissue, HEK-293 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inT-47D cells, A431 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17565-1-AP targets Beta Catenin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Catenin fusion protein Ag11763
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC058926
Gene symbol CTNNB1
Gene ID (NCBI) 1499
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Protocols

Product Specific Protocols
WB protocol for Beta Catenin antibody 17565-1-APDownload protocol
IHC protocol for Beta Catenin antibody 17565-1-APDownload protocol
IF protocol for Beta Catenin antibody 17565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

J Cell Physiol

WISP1 silencing confers protection against epithelial-mesenchymal transition of renal tubular epithelial cells in rats via inactivation of the wnt/β-catenin signaling pathway in uremia.

Authors - Yuan-Zhen Chen
mouseIF

J Control Release

Alginate/Chitosan microparticles for gastric passage and intestinal release of therapeutic protein nanoparticles.

Authors - Kevin Ling
humanWB

Biochem Cell Biol

The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway.

Authors - Sijia Han
humanWB

Cancer Sci

LncRNA MIR22HG inhibits growth, migration and invasion through regulating the miR-10a-5p/NCOR2 axis in hepatocellular carcinoma cells.

Authors - Yangjun Wu
ratIF

In Vitro Cell Dev Biol Anim

miR-145-5p suppresses osteogenic differentiation of adipose-derived stem cells by targeting semaphorin 3A.

Authors - Xiangdong Liu
humanWB

Eur Rev Med Pharmacol Sci

MiR-202-3p functions as a tumor suppressor and reduces cell migration and invasion in papillary thyroid carcinoma.

Authors - J Chen