beta-Catenin Antibody 17565-1-AP

Tested Applications

Applications:

ELISA, IF, IHC, WB
Application not tested? Please see our Guarantee

A431 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

Immunofluorescent analysis of A431 cells using 17565-1-AP(beta-Catenin antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17565-1-AP( beta-Catenin antibody at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17565-1-AP( beta-Catenin antibody at dilution of 1:50 (under 40x lens)

Species specificity:

human, mouse, rat, zebrafish

Positive WB detected in:

A431 cells, mouse brain tissue, NIH/3T3 cells, rat brain tissue, HeLa cells, MCF-7 cells, mouse liver tissue, HEK-293 cells

Positive IHC detected in:

human breast cancer tissue

Positive IF detected in:

A431 cells, MCF-7 cells

Recommended dilution:

WB : 1:500-1:1000

IHC : 1:50-1:500

IF : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

beta-Catenin fusion protein Ag11763

Full name:

catenin (cadherin-associated protein), beta 1, 88kDa

Calculated molecular weight:

781aa,86 kDa

Observed molecular weight:

92 kDa

GenBank accession number:

BC058926

Gene ID (NCBI):

1499

Gene symbol

CTNNB1

Synonyms

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286, β catenin, β-catenin

Background

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Showing 1- [2] of [2] Publications

Author:		Yang Song
Pubmed ID:		27542262
Journal:		Oncotarget
Title:		Elevated eukaryotic elongation factor 2 expression is involved in proliferation and invasion of lung squamous cell carcinoma.
Application:		WB

Species:		human

Author:		Jianyun Zhu
Pubmed ID:		28431267
Journal:		J Nutr Biochem
Title:		miR-19 targeting of GSK3β mediates sulforaphane suppression of lung cancer stem cells.
Application:		WB

Species:		human

WB Figures

A431 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

HEK-293 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

HeLa cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

MCF-7 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

mouse brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night

mouse liver tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP( beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17565-1-AP( beta-Catenin antibody at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17565-1-AP( beta-Catenin antibody at dilution of 1:50 (under 40x lens)

IF Figures

Immunofluorescent analysis of A431 cells using 17565-1-AP(beta-Catenin antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of MCF-7 cells using 17565-1-AP(beta-Catenin antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

beta-Catenin Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 17565-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [2] of [2] Publications

WB Figures

IHC Figures

IF Figures

beta-Catenin Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 17565-1-AP

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Special offer: Add control/tag antibody for $99

Tested Applications

Product Information

Immunogen Information

Background