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Beta Catenin Polyclonal antibody (17565-1-AP)

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KD/KO Validated

Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 17565-1-AP

Publications(174)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Synonyms

b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

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Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 17565-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17565-1-AP (Beta Catenin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 17565-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 17565-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat brain using 17565-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of NIH/3T3 using 17565-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HEK-293 using 17565-1-AP
IP result of anti-Beta Catenin (IP:17565-1-AP, 4ug; Detection:17565-1-AP 1:5000) with HEK-293 cells lysate 1920 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 17565-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 17565-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 17565-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17565-1-AP (Beta Catenin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 17565-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17565-1-AP (Beta Catenin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 17565-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 17565-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse colon using 17565-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of rat pancreas using 17565-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat pancreas tissue using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse liver using 17565-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of U-251 using 17565-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 17565-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of U-87 MG using 17565-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 17565-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of T-47D using 17565-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of MCF-7 using 17565-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Beta Catenin (17565-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (174)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells, rat brain tissue, HeLa cells, C6 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human liver cancer tissue, human breast cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse colon tissue, rat pancreas tissue, mouse liver tissue
Positive IF/ICC detected in	MCF-7 cells, T-47D cells, U-251 cells, U-87 MG cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17565-1-AP targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11763 Product name: Recombinant human B-catenin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 433-781 aa of BC058926 Sequence: KNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL Predict reactive species
Full Name	catenin (cadherin-associated protein), beta 1, 88kDa
Calculated Molecular Weight	781 aa, 86 kDa
Observed Molecular Weight	92 kDa
GenBank Accession Number	BC058926
Gene Symbol	Beta Catenin
Gene ID (NCBI)	1499
ENSEMBL Gene ID	ENSG00000168036
RRID	AB_2088102
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35222
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to α-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases, including cancers. (PMID: 22617422; 18334222)

Protocols

Product Specific Protocols
FC protocol for Beta Catenin antibody 17565-1-AP	Download protocol
IF protocol for Beta Catenin antibody 17565-1-AP	Download protocol
IHC protocol for Beta Catenin antibody 17565-1-AP	Download protocol
IP protocol for Beta Catenin antibody 17565-1-AP	Download protocol
WB protocol for Beta Catenin antibody 17565-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB,IP	Cell Dual-specificity RNA aptamers enable manipulation of target-specific O-GlcNAcylation and unveil functions of O-GlcNAc on β-catenin Authors - Yi Zhu View Article
mouse	WB	Nat Commun Decreased scleral Wnt5a(hi) fibroblasts exacerbate myopia progression by disrupting extracellular matrix homeostasis in mice. Authors - He Zhu View Article
rat	IF	ACS Nano Three-Dimensional Bioprinted Scaffolds Loaded with Multifunctional Magnesium-Based Metal-Organic Frameworks Improve the Senescence Microenvironment Prompting Aged Bone Defect Repair. Authors - Xin Sun View Article
		J Nanobiotechnology Nanoparticle-integrated smart hydrogel with H(2)S-triggered antibacterial escalation and sustained oxygen release for periodontal regeneration. Authors - Jingshu Yang View Article
mouse	WB,IF	J Clin Invest JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling. Authors - Yaping Zhang View Article
human	WB	J Exp Clin Cancer Res PMID: 31319849 Authors - Ling Deng View Article