GST tag Monoclonal antibody

GST tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, schistosoma japonicum

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3B5F8

Cat no : 66001-1-Ig

Synonyms



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66001-1-Ig targets GST tag in WB, IP, CoIP, ELISA applications and shows reactivity with recombinant protein, schistosoma japonicum samples.

Tested Reactivity recombinant protein, schistosoma japonicum
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name GST tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.

Publications

SpeciesApplicationTitle

Science

A LIMA1 variant promotes low plasma LDL cholesterol and decreases intestinal cholesterol absorption.

Authors - Ying-Yu Zhang

J Clin Invest

Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1.

Authors - Gaoxiang Zhao

Sci Adv

Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells.

Authors - Xiangxiang Wei

Nat Cell Biol

AMPK-mediated activation of MCU stimulates mitochondrial Ca2+ entry to promote mitotic progression.

Authors - Haixin Zhao

J Exp Clin Cancer Res

CyclinD1 inhibits dicer and crucial miRNA expression by chromatin modification to promote the progression of intrahepatic cholangiocarcinoma.

Authors - Yongqiang Qi

Mol Plant Pathol

An effector of a necrotrophic fungal pathogen targets the calcium-sensing receptor in chloroplasts to inhibit host resistance.

Authors - Liguang Tang