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GST tag Antibody 31 Publications

Mouse Monoclonal| Catalog number: 66001-1-Ig |CloneNo.: 3B5F8

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Con: 205 μg/150 μl

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Tested Applications

Applications:
WB, IP, ELISA
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Species specificity:
recombinant protein, schistosoma japonicum, human
Recommended dilution:
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Recombinant Protein
Full name:
GST tag
Calculated molecular weight:
26 kDa
Observed molecular weight:
GenBank accession number:
FN315687
Gene ID (NCBI):
Gene symbol
Synonyms

Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.


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