GST tag Monoclonal antibody
GST tag Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein, schistosoma japonicum
Applications
WB, IP, CoIP,ELISA
Conjugate
Unconjugated
CloneNo.
3B5F8
Cat no : 66001-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 11 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
Product Information
66001-1-Ig targets GST tag in WB, IP, CoIP,ELISA applications and shows reactivity with recombinant protein, schistosoma japonicum samples.
Tested Reactivity | recombinant protein, schistosoma japonicum |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant Protein |
Full Name | GST tag |
Calculated molecular weight | 26 kDa |
GenBank accession number | FN315687 |
Gene symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.
Publications
Species | Application | Title |
---|---|---|
Science A LIMA1 variant promotes low plasma LDL cholesterol and decreases intestinal cholesterol absorption. | ||
J Clin Invest Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1. | ||
Sci Adv Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells. | ||
Nat Cell Biol AMPK-mediated activation of MCU stimulates mitochondrial Ca2+ entry to promote mitotic progression. | ||
J Exp Clin Cancer Res CyclinD1 inhibits dicer and crucial miRNA expression by chromatin modification to promote the progression of intrahepatic cholangiocarcinoma. | ||
Mol Plant Pathol An effector of a necrotrophic fungal pathogen targets the calcium-sensing receptor in chloroplasts to inhibit host resistance. |