AMACR/p504S Polyclonal antibody

AMACR/p504S Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15918-1-AP

Synonyms

2 methylacyl CoA racemase, alpha methylacyl CoA racemase, AMACR, AMACR/p504S, CBAS4, p504S, RACE



Tested Applications

Positive WB detected inmulti-cells, LNCaP cells
Positive IHC detected inhuman prostate cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 15918-1-AP is AMACR/p504S Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMACR/p504S fusion protein Ag8720
Full Name alpha-methylacyl-CoA racemase
Calculated Molecular Weight 382 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC009471
Gene Symbol AMACR
Gene ID (NCBI) 23600
RRIDAB_11126973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMACR(Alpha-methylacyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing ang it can form a a dimer of 70-75 kDa(PMID:21812041). Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

Protocols

Product Specific Protocols
WB protocol for AMACR/p504S antibody 15918-1-APDownload protocol
IHC protocol for AMACR/p504S antibody 15918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang
humanIHC

Front Cell Dev Biol

SMARCC1 Suppresses Tumor Progression by Inhibiting the PI3K/AKT Signaling Pathway in Prostate Cancer.

Authors - Zhao-Ming Xiao
humanIHC

Oncol Lett

The auxiliary diagnostic value of prostate-specific antigen and α-methylacyl-CoA racemase in prostate cancer.

Authors - Daijun Yang
humanIHC

Andrologia

Circ_0076305 facilitates prostate cancer development via sponging miR-411-5p and regulating PGK1.

Authors - Xiqi Ding