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p70(S6K) Antibody 39 Publications

Rabbit Polyclonal| Catalog number: 14485-1-AP

Featured Product KD/KO validated

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Con: 32 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

p70(S6K) Antibody WB HeLa cells 14485-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 14485-1-AP(p70S6K antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours

p70(S6K) Antibody IP HeLa cells 14485-1-AP

IP Result of anti-p70S6K (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.

p70(S6K) Antibody IHC human breast cancer tissue 14485-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14485-1-AP( p70S6K antibody at dilution of 1:200 (under 10x lens).

p70(S6K) Antibody IHC human breast cancer tissue 14485-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14485-1-AP( p70S6K antibody at dilution of 1:200 (under 40x lens).

p70(S6K) Antibody IF HepG2 cells 14485-1-AP

Immunofluorescent analysis of HepG2 cells using 14485-1-AP(p70S6K antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

p70(S6K) Antibody FC HepG2 cells 14485-1-AP

1X10^6 HepG2 cells were stained with 0.2ug p70S6K antibody (14485-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat, blunt snout bream
Positive WB detected in:
HeLa cells, HepG2 cells, Jurkat cells, rat spleen tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2400
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:100-1:400
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
p70(S6K) fusion protein Ag5883
Full name:
ribosomal protein S6 kinase, 70kDa, polypeptide 1
Calculated molecular weight:
59 kDa
Observed molecular weight:
70 kDa
GenBank accession number:
BC053365
Gene ID (NCBI):
6198
Gene symbol
RPS6KB1
Synonyms
p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K) alpha, p70S6K, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A

Background

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating insulin sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59 kDa produced by alternative initiation.


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