-Antibody-14485-1-AP-WB-268098.jpg "WB analysis of MCF-7 using 14485-1-AP")
-Antibody-14485-1-AP-WB-428140.jpg "WB analysis of MCF-7 using 14485-1-AP")
-Antibody-14485-1-AP-WB-484367.jpg "WB analysis using 14485-1-AP")
-Antibody-14485-1-AP-WB-180462.jpg "WB analysis using 14485-1-AP")
-Antibody-14485-1-AP-IP-35809.jpg "IP experiment of HeLa using 14485-1-AP")
-Antibody-14485-1-AP-IHC-252307.jpg "IHC staining of human liver cancer using 14485-1-AP")
-Antibody-14485-1-AP-IF-17645.jpg "IF Staining of HepG2 using 14485-1-AP")
-Antibody-14485-1-AP-IF-408919.jpg "IF Staining of HepG2 using 14485-1-AP")
Tested Applications
| Positive WB detected in | HEK-293 cells, IGF-1 treated MCF-7 cells, Jurkat cells, NIH/3T3 cells, HeLa cells, PC-12 cells, C6 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14485-1-AP targets p70(S6K) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, chicken, bovine, megalobrama amblycephala |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5883 Product name: Recombinant human p70(S6K) protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC053365 Sequence: MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPF Predict reactive species |
| Full Name | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 65-75 kDa, 80-85 kDa |
| GenBank Accession Number | BC053365 |
| Gene Symbol | p70 S6K |
| Gene ID (NCBI) | 6198 |
| RRID | AB_2269787 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23443 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA, and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of mTOR and acts as a crucial effector of mTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K). The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation, and metabolism and is the central regulator of translation of some components of the protein synthesis system. Due to alternative translation, two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IHC protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IP protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| WB protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The p70(S6K) polyclonal antibody (Cat# 14485-1-AP) has 274 total citations published in journals including Nature, Science, Cell Metabolism, Molecular Cancer, Autophagy, Oncogene, Advanced Science, Cell Death & Differentiation, and Nature Communications. Associated research fields encompass mTOR signaling, autophagy, cancer biology, metabolism, inflammation, neuroscience, cardiology, and immunology. The antibody is primarily used for Western blotting in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther A CRISPR knockout negative screen reveals synergy between CDKs inhibitor and metformin in the treatment of human cancer in vitro and in vivo. | ||
Science Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling | ||
Mol Cancer Role of the lncRNA ABHD11-AS1 in the tumorigenesis and progression of epithelial ovarian cancer through targeted regulation of RhoC. | ||
Mol Cancer S6K1 amplification confers innate resistance to CDK4/6 inhibitors through activating c-Myc pathway in patients with estrogen receptor-positive breast cancer
|
Reviews
What customers say
Two reviewers tested this antibody on HeLa cells at a 1:100 dilution. One user (Oct 2024) gave it 5/5 stars, reporting it worked well for immunofluorescence. However, another user (Aug 2025) rated it 2/5, stating it did not work for either Western Blot or Immunofluorescence. Overall, feedback is mixed, with one positive experience in IF and one unsuccessful attempt across both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tabassum (Verified Customer) (08-21-2025) | Unfortunately this didn’t work for my western and Immunoflurescence
|
Tabassum (Verified Customer) (10-17-2024) | It was overall good.
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