-Antibody-14485-1-AP-WB-268098.jpg "MCF-7 cells were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.")
-Antibody-14485-1-AP-WB-428140.jpg "WB result of p70(S6K) antibody (14485-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p70(S6K) transfected MCF-7 cells.")
-Antibody-14485-1-AP-WB-484367.jpg "Various lysates were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.")
-Antibody-14485-1-AP-WB-180462.jpg "Various lysates were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.")
-Antibody-14485-1-AP-IP-35809.jpg "IP result of anti-p70(S6K) (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.")
-Antibody-14485-1-AP-IHC-252307.jpg "Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14485-1-AP (p70(S6K) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).")
-Antibody-14485-1-AP-IF-17645.jpg "Immunofluorescent analysis of HepG2 cells using 14485-1-AP (p70S6K antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.")
-Antibody-14485-1-AP-IF-408919.jpg "Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p70(S6K) antibody (14485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).")
Tested Applications
| Positive WB detected in | HEK-293 cells, IGF-1 treated MCF-7 cells, Jurkat cells, NIH/3T3 cells, HeLa cells, PC-12 cells, C6 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 195 publications below |
| IHC | See 5 publications below |
| IF | See 3 publications below |
Product Information
14485-1-AP targets p70(S6K) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, megalobrama amblycephala |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | p70(S6K) fusion protein Ag5883 Predict reactive species |
| Full Name | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 65-75 kDa, 80-85 kDa |
| GenBank Accession Number | BC053365 |
| Gene Symbol | p70 S6K |
| Gene ID (NCBI) | 6198 |
| RRID | AB_2269787 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23443 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA, and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of mTOR and acts as a crucial effector of mTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K). The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation, and metabolism and is the central regulator of translation of some components of the protein synthesis system. Due to alternative translation, two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IHC protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IF protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IP protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis. | ||
Sci Adv Citrulline regulates macrophage metabolism and inflammation to counter aging in mice | ||
Small Methods PROTAC Degraders of Androgen Receptor-Integrated Dissolving Microneedles for Androgenetic Alopecia and Recrudescence Treatment via Single Topical Administration | ||
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Redox Biol Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tabassum (Verified Customer) (10-17-2024) | It was overall good.
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