HeLa cells were subjected to SDS PAGE followed by western blot with 14485-1-AP(p70S6K antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 14485-1-AP(p70S6K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 14485-1-AP(p70S6K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

rat spleen tissue were subjected to SDS PAGE followed by western blot with 14485-1-AP( p70S6K antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14485-1-AP( p70S6K antibody at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14485-1-AP( p70S6K antibody at dilution of 1:200 (under 40x lens).

Immunohistochemical of paraffin-embedded human breast cancer using 14485-1-AP(p70S6K antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using 14485-1-AP(p70S6K antibody) at dilution of 1:100 (under 40x lens)

IP Result of anti-p70S6K (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.

Immunofluorescent analysis of HepG2 cells using 14485-1-AP(p70S6K antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

1X10^6 HepG2 cells were stained with 0.2ug p70S6K antibody (14485-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.