• Featured Product
  • KD/KO Validated

p70(S6K) Polyclonal antibody

p70(S6K) Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14485-1-AP

Synonyms

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K) alpha, p70S6K, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, rat spleen tissue, PC-12 cells, C6 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14485-1-AP targets p70(S6K) in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity blunt snout bream, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p70(S6K) fusion protein Ag5883
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Calculated molecular weight 59 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC053365
Gene symbol RPS6KB1
Gene ID (NCBI) 6198
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59 kDa produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for p70(S6K) antibody 14485-1-APDownload protocol
IHC protocol for p70(S6K) antibody 14485-1-APDownload protocol
IF protocol for p70(S6K) antibody 14485-1-APDownload protocol
IP protocol for p70(S6K) antibody 14485-1-APDownload protocol
FC protocol for p70(S6K) antibody 14485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Onco Targets Ther

XRCC1 rs1799782 (C194T) polymorphism correlated with tumor metastasis and molecular subtypes in breast cancer.

Authors - Qing Li
blunt snout breamWB

Fish Physiol Biochem

Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala.

Authors - Hualiang Liang
humanWB

Oncol Lett

JTC-801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3-kinase/protein kinase B signalling pathway.

Authors - Bufei Zhao
humanWB

J Genet Genomics

mTOR-S6K1 pathway mediates cytoophidium assembly.

Authors - Zhe Sun
mouseWB

Exp Ther Med

Role of mTOR signaling in the regulation of high glucose-induced podocyte injury.

Authors - Qiuyue Li
ratWB

Cell Tissue Res

Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears.

Authors - Rongzong Zheng