- Featured Product
- KD/KO Validated
p70(S6K) Polyclonal antibody
p70(S6K) Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 14485-1-AP
Synonyms
Validation Data Gallery
-Antibody-14485-1-AP-WB-180462.jpg "Various lysates were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.")
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-Antibody-14485-1-AP-IP-35809.jpg "IP Result of anti-p70S6K (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.")
-Antibody-14485-1-AP-IP-35809.jpg)
-Antibody-14485-1-AP-IHC-73219.jpg "Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14485-1-AP (p70S6K antibody at dilution of 1:200 (under 10x lens).")
-Antibody-14485-1-AP-IHC-73219.jpg)
-Antibody-14485-1-AP-IHC-73220.jpg "Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14485-1-AP (p70S6K antibody at dilution of 1:200 (under 40x lens).")
-Antibody-14485-1-AP-IHC-73220.jpg)
-Antibody-14485-1-AP-IF-17645.jpg "Immunofluorescent analysis of HepG2 cells using 14485-1-AP (p70S6K antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.")
-Antibody-14485-1-AP-IF-17645.jpg)
-Antibody-14485-1-AP-FC-38056.jpg "1X10^6 HepG2 cells were stained with 0.2ug p70S6K antibody (14485-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.")
-Antibody-14485-1-AP-FC-38056.jpg)
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, rat spleen tissue, PC-12 cells, C6 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 60 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
14485-1-AP targets p70(S6K) in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | blunt snout bream, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | p70(S6K) fusion protein Ag5883 |
| Full Name | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC053365 |
| Gene symbol | RPS6KB1 |
| Gene ID (NCBI) | 6198 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59 kDa produced by alternative initiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IHC protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IF protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IP protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| FC protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Onco Targets Ther XRCC1 rs1799782 (C194T) polymorphism correlated with tumor metastasis and molecular subtypes in breast cancer. | ||
Fish Physiol Biochem Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala. | ||
Oncol Lett JTC-801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3-kinase/protein kinase B signalling pathway. | ||
Exp Ther Med Role of mTOR signaling in the regulation of high glucose-induced podocyte injury. | ||
Cell Tissue Res Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears. |
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