Pan-PAX Polyclonal antibody

Pan-PAX Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21383-1-AP

Synonyms

paired box, paired box 8, Paired box protein Pax, Paired box protein Pax 8, Pan-PAX, PAX 8, PAX8



Tested Applications

Positive WB detected inSKOV-3 cells, A2780 cells
Positive IP detected inSKOV-3 cells
Positive IHC detected inhuman ovary tumor tissue, human renal cell carcinoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21383-1-AP is Pan-PAX Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Pan-PAX fusion protein Ag16018
Full Name paired box 8
Calculated molecular weight 450 aa, 48 kDa
Observed molecular weight 36-48 kDa
GenBank accession numberBC001060
Gene symbol PAX8
Gene ID (NCBI) 7849
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX genes encode a family of developmentally regulated transcription factors that have been implicated in a number of human and murine congenital disorders, as well as in tumorigenesis. These genes are defined by the presence of an evolutionarily conserved DNA binding domain, termed the paired domain. Many paired domain-containing proteins recognize a common sequence motif. The immunogen of this atnibody is the Paired box domain of PAX family. This antibody is a Pan-PAX antibody.

Protocols

Product Specific Protocols
WB protocol for Pan-PAX antibody 21383-1-APDownload protocol
IHC protocol for Pan-PAX antibody 21383-1-APDownload protocol
IF protocol for Pan-PAX antibody 21383-1-APDownload protocol
IP protocol for Pan-PAX antibody 21383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Life Sci

Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy.

Authors - Qiwang Ma
pigIF

PLoS One

PMID: 28231273

Authors - Sergio E Palma-Vera
mouseIHC

Endocrine

PMID: 32281047

Authors - Mika Shimamura
humanIHC

Am J Surg Pathol

PAX8 and PAX2 immunostaining facilitates the diagnosis of primary epithelial neoplasms of the male genital tract.

Authors - Tong Guo-Xia GX
ratIHC

Sci Signal

Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.

Authors - Zi-Yang Zhang