Phospho-TDP43 (Ser403/404) Monoclonal antibody

Phospho-TDP43 (Ser403/404) Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

6B11B12

Cat no : 66079-1-Ig

Synonyms

ALS10, Phospho-TDP43 (Ser403/404), TAR DNA binding protein, TAR DNA binding protein 43, TDP 43, TDP43



Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, RAW 264.7 cells, fetal human brain tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

66079-1-Ig targets Phospho-TDP43 (Ser403/404) in WB, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 25 kDa
GenBank accession numberNM_007375
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).66079-1-Ig is a mouse monoclonal antibody recognizing TDP-43 only when phosphorylated at 403/404. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Protocols

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser403/404) antibody 66079-1-IgDownload protocol
FC protocol for Phospho-TDP43 (Ser403/404) antibody 66079-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Drosophila lines with mutant and wild type human TDP-43 replacing the endogenous gene reveals phosphorylation and ubiquitination in mutant lines in the absence of viability or lifespan defects.

Authors - Jer-Cherng Chang
humanIF

Biochemistry

Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.

Authors - Janice S W Ng