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phospho(409/410)-TDP43 Antibody 11 Publications

Rabbit Polyclonal| Catalog number: 22309-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

phospho(409/410)-TDP43 Antibody WB Ethacrynic acid treated HeLa cells 22309-1-AP

Ethacrynic acid treated HeLa cells were subjected to SDS PAGE followed by western blot with 22309-1-AP (phospho(409/410)-TDP43 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

phospho(409/410)-TDP43 Antibody WB human placenta tissue 22309-1-AP

human placenta tissue were subjected to SDS PAGE followed by western blot with 22309-1-AP(phospho(409/410)-TDP43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse
Positive WB detected in:
Ethacrynic acid treated HeLa cells, mouse heart tissue, human heart tissue, human placenta tissue
Recommended dilution:
WB : 1:300-1:1000

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
TAR DNA binding protein
Calculated molecular weight:
43 kDa
Observed molecular weight:
45-50, 25-35 kDa
GenBank accession number:
NM_007375
Gene ID (NCBI):
23435
Gene symbol
TARDBP
Synonyms
phospho TDP43, phospho(409/410)-TDP43, TARDBP, TDP43

Background

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. This antibody detects phosphorylated forms of TDP-43 as well as the cleavage product of 20-30 kDa. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.


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