Phospho-TDP43 (Ser409/410) Polyclonal antibody

Phospho-TDP43 (Ser409/410) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22309-1-AP

Synonyms

phospho TDP43, phospho(409/410)-TDP43, Phospho-TDP43 (Ser409/410), TARDBP, TDP43



Tested Applications

Positive WB detected inEthacrynic acid treated HeLa cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Sample-dependent, check data in validation data gallery

Product Information

22309-1-AP targets Phospho-TDP43 (Ser409/410) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 45-50 kDa, 25-35 kDa
GenBank accession numberNM_007375
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Protocols

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 22309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Biol Chem

Aggregation of the nucleic acid-binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation.

Authors - Youjun Chen
humanWB,IHC,IF

Lab Invest

Association between TDP-43 and mitochondria in inclusion body myositis.

Authors - Mikayla L Huntley

J Alzheimers Dis

Phospho-Tau Changes in the Human CA1 During Alzheimer's Disease Progression.

Authors - Mamen Regalado-Reyes

Future Sci OA

Platelet phosphorylated TDP-43: an exploratory study for a peripheral surrogate biomarker development for Alzheimer's disease.

Authors - Rodger Wilhite
humanIHC,IF

Acta Neuropathol Commun

Phosphorylated TDP-43 (pTDP-43) aggregates in the axial skeletal muscle of patients with sporadic and familial amyotrophic lateral sclerosis.

Authors - Matthew D Cykowski
humanIHC

Acta Neuropathol Commun

Fragile X-associated tremor ataxia syndrome with co-occurrent progressive supranuclear palsy-like neuropathology.

Authors - Amanda N Sacino

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (03-10-2020)

Immunofluorescent analysis of (4% PFA fixed) NSC-34 cells, using p- (109/110) TDP-43 antibody at 1/500 dilution and Alexa Flour conjugated Donkey anti Rabbit IgG (H+L) (Red). Bisbenzimide (Blue) labels cell nuclei.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: NSC-34 motor neuron like cell line
Phospho-TDP43 (Ser409/410) Antibody Immunofluorescence, validation (1/500 dilution) in NSC-34 motor neuron like cell line (Cat no:22309-1-AP)
FH

Laura (Verified Customer) (01-10-2020)

Works well at this concentration on HEK293T lysate.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
FH

Owen (Verified Customer) (10-30-2019)

This antibody was used to image TDP43 in tissue and cell samples. The antibody was useful to image such a difficult target.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Human cortical tissue / motor neurons