Phospho-AKT (Tyr315) Monoclonal antibody
Phospho-Akt (Tyr315) Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
5C4D12
Cat no : 60072-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
| IF | See 1 publications below |
Product Information
60072-1-Ig targets Phospho-Akt (Tyr315) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Phospho Peptide |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Calculated molecular weight | 56 kDa |
| Observed molecular weight | 62 kDa |
| GenBank accession number | NM_005163 |
| Gene symbol | AKT1 |
| Gene ID (NCBI) | 207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein kinase B (PKB/Akt) has been well established as an important signaling intermediate, and its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337). Akt, also known as protein kinase B (PKB), is a serine/threonine kinase activated downstream of class I phosphatidylinositol 3-kinase (PI3K) and various receptors. It has emerged as the key regulator for many signal transduction pathways, modulating multiple processes such as cell survival, proliferation, growth, angiogenesis, and glucose metabolism(PMID:16139227). Its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337).
Publications
| Species | Application | Title |
|---|---|---|
Exp Ther Med Protective effects of p-nitro caffeic acid phenethyl ester on acute myocardial ischemia-reperfusion injury in rats. | ||
Nutrients Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats. | ||
Int Immunopharmacol Low dose photodynamic-therapy induce immune escape of tumor cells in a HIF-1α dependent manner through PI3K/Akt pathway. | ||
Int J Mol Sci Sinulariolide Suppresses Human Hepatocellular Carcinoma Cell Migration and Invasion by Inhibiting Matrix Metalloproteinase-2/-9 through MAPKs and PI3K/Akt Signaling Pathways. | ||
Chem Biol Interact SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells. | ||
Cell Physiol Biochem PRDX6 Protects ARPE-19 Cells from Oxidative Damage via PI3K/AKT Signaling. |