Phospho-Akt (Tyr315) Antibody

Phospho-Akt (Tyr315) Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5C4D12

Cat no : 60072-1-Ig

Synonyms



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60072-1-Ig targets Phospho-Akt (Tyr315) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Phospho Peptide
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight 62 kDa
GenBank accession number NM_005163
Gene symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase B (PKB/Akt) has been well established as an important signaling intermediate, and its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337). Akt, also known as protein kinase B (PKB), is a serine/threonine kinase activated downstream of class I phosphatidylinositol 3-kinase (PI3K) and various receptors. It has emerged as the key regulator for many signal transduction pathways, modulating multiple processes such as cell survival, proliferation, growth, angiogenesis, and glucose metabolism(PMID:16139227). Its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337).

Publications

SpeciesApplicationTitle
ratWB

Exp Ther Med

Protective effects of p-nitro caffeic acid phenethyl ester on acute myocardial ischemia-reperfusion injury in rats.

Authors - Qin DU
ratWB

Nutrients

Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats.

Authors - Deshi Dong
humanWB

Int Immunopharmacol

Low dose photodynamic-therapy induce immune escape of tumor cells in a HIF-1α dependent manner through PI3K/Akt pathway.

Authors - Jun Wan
humanWB

Int J Mol Sci

Sinulariolide Suppresses Human Hepatocellular Carcinoma Cell Migration and Invasion by Inhibiting Matrix Metalloproteinase-2/-9 through MAPKs and PI3K/Akt Signaling Pathways.

Authors - Yu-Jen Wu
humanWB

Chem Biol Interact

SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.

Authors - Jingjun Wu
IF

Cell Physiol Biochem

PRDX6 Protects ARPE-19 Cells from Oxidative Damage via PI3K/AKT Signaling.

Authors - Xu Zha