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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human VEGF165

Activity

Typically ≤ 5 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1038

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF165, VEGFA, VPF



Technical Specifications

GeneID 7422
Species human
Expression HEK293
Activity Typically ≤ 5 ng/mL EC50
Purity >95%
Molecular Mass 45 kDa, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant serum albumin (HSA).

Product Description

Animal-free Recombinant Human VEGF 165is expressed in human 293 cells as a homodimeric glycoprotein with an apparent molecular mass of 45 kDa. The E. coli expressed protein is a monomer-dimer mixture with a molecular mass 18 to 34 kDa. The cytokine is produced in a human cell expression system with serum free, chemically defined media. VEGF, also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34-45 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic, and vascular permeability enhancing activities specific for endothelial cells.

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer (PMID 10838264; 11329058). This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulations of this gene in mice result in abnormal embryonic blood vessel formation.

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF165, VEGFA, VPF

Publications

SpeciesTitle
human

J Exp Clin Cancer Res

Receptor tyrosine kinase inhibitor Sunitinib and integrin antagonist peptide HM-3 show similar lipid raft dependent biphasic regulation of tumor angiogenesis and metastasis.

Authors - Jialiang Hu
human

J Biosci Bioeng

Construction of 3D cardiac tissue with synchronous powerful beating using human cardiomyocytes from human iPS cells prepared by a convenient differentiation method.

Authors - Yumi Sasano
human

Cell Mol Biol Lett

ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model.

Authors - Yasuhiro Yoshida
human

Int J Neurosci

Vascular endothelial growth factor alleviates mitochondrial dysfunction and suppression of mitochondrial biogenesis in models of Alzheimer's disease.

Authors - Xiangtian Liu
human

Wound Repair Regen

Vascular endothelial growth factor promotes macrophage apoptosis through stimulation of tumor necrosis factor superfamily member 14 (TNFSF14/LIGHT).

Authors - Melissa L Petreaca
human

J Tissue Eng Regen Med

Noninvasive OCT Imaging of Three-Dimensional Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells.

Authors - Atsushi Miyaoka