4EBP1 Monoklonaler Antikörper

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• KD/KO Validated

4EBP1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(68)

CloneNo.

2C3F3

Rezensionen (3)

Kat-Nr. : 60246-1-Ig

Synonyme

EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1

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  WB analysis of K562 using 60246-1-Ig

  WB result of 4EBP1 antibody (60246-1-Ig, 1:4000) with si-Control and si-4EBP1 transfected K562 cells.

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  WB analysis of K-562 using 60246-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 60246-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 60246-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 60246-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60246-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60246-1-Ig(4EBP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HCT 116-Zellen, K562, NCI-H1299-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 63 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

60246-1-Ig bindet in WB, IHC, IF/ICC, ELISA 4EBP1 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Huhn
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: 4EBP1 fusion protein Ag18985
• Vollständiger Name: eukaryotic translation initiation factor 4E binding protein 1
• Berechnetes Molekulargewicht: 118 aa, 12 kDa
• Beobachtetes Molekulargewicht: 15-24 kDa
• GenBank-Zugangsnummer: BC004459
• Gene symbol: EIF4EBP1
• Gene ID (NCBI): 1978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)

Publikationen

Species	Application	Title
	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
		J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
human	IHC	Mol Cancer; The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation.; Authors - Liang-Yan Chen
	WB	Sci Adv; Citrulline regulates macrophage metabolism and inflammation to counter aging in mice; Authors - Zhangdan Xie
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Autophagy; STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.; Authors - Cefan Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (12-06-2024)

EIF4EBP1 worked well enugh in my FFPE human cortex marking my neurons (in red)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

4EBP1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:60246-1-Ig)

FH

Tom (Verified Customer) (03-02-2021)

10ug of HEK293 lysate resolved on 4-20% gradient acrylamide gel. Membrane blocked (5% BSA) then incubated overnight with antibody (1:2,000). Incubated with secondary HRP antibody for visulization.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293

4EBP1 Antibody Western Blot validation (1:2000 dilution) in HEK293 (Cat no:60246-1-Ig)

FH

Yifei (Verified Customer) (11-01-2017)

It works well for Western blot

• Applications: Western Blot