Alpha-2-Macroglobulin Polyklonaler Antikörper

Alpha-2-Macroglobulin Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 13545-1-AP

Synonyme

A2M, C3 and PZP like alpha 2 macroglobulin domain containing protein 5, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin

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  WB analysis of human plasma using 13545-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13545-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13545-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13545-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13545-1-AP

  IP result of anti-Alpha-2-Macroglobulin (IP:13545-1-AP, 4ug; Detection:13545-1-AP 1:2000) with HepG2 cells lysate 1080 ug.

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  IHC staining of human liver using 13545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 13545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 13545-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13545-1-AP (Alpha-2-Macroglobulin antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13545-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (13545-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe, HepG2-Zellen, humanes Hirngewebe, humanes Plasmagewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

13545-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Alpha-2-Macroglobulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Plasmodium falciparum
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha-2-Macroglobulin fusion protein Ag4489
• Vollständiger Name: alpha-2-macroglobulin
• Berechnetes Molekulargewicht: 1474 aa, 163 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC040071
• Gene symbol: Alpha 2 Macroglobulin
• Gene ID (NCBI): 2
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Biometal Dyshomeostasis in Olfactory Mucosa of Alzheimer's Disease Patients.; Authors - Riikka Lampinen
human	IF	Am J Cancer Res; Prognostic significance of alpha-2-macrglobulin and low-density lipoprotein receptor-related protein-1 in various cancers; Authors - Mateusz Olbromski
Plasmodium falciparum	WB	Malar J; The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.; Authors - Takahiro Tougan
human	IF	Int J Biochem Cell Biol; Restoration of A2M reduces drug resistance and malignancy in paclitaxel-resistant lung cancer cells; Authors - Xiaona Fan