Alpha-2-Macroglobulin Monoklonaler Antikörper

Alpha-2-Macroglobulin Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

4B11F7

Kat-Nr. : 66126-1-Ig

Synonyme

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M

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  WB analysis of HepG2 using 66126-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66126-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 66126-1-Ig

  IP result of anti-Alpha-2-Macroglobulin (IP:66126-1-Ig, 5ug; Detection:66126-1-Ig 1:500) with human plasma lysate 4000ug.

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  IHC staining of human liver using 66126-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66126-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66126-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (66126-1-Ig, Clone: 4B11F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Hirngewebe
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:32000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

66126-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA Alpha-2-Macroglobulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alpha-2-Macroglobulin fusion protein Ag19245
• Vollständiger Name: alpha-2-macroglobulin
• Berechnetes Molekulargewicht: 1474 aa, 163 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC040071
• Gene symbol: Alpha 2 Macroglobulin
• Gene ID (NCBI): 2
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Publikationen

Species	Application	Title
human	IHC	Am J Cancer Res; Prognostic significance of alpha-2-macrglobulin and low-density lipoprotein receptor-related protein-1 in various cancers; Authors - Mateusz Olbromski
mouse	WB	Front Physiol; Proteomic analyses of urinary exosomes identify novel potential biomarkers for early diagnosis of sickle cell nephropathy, a sex-based study; Authors - Balamurugan Packialakshmi