ABCD1 Polyklonaler Antikörper

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• KD/KO Validated

ABCD1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 18138-1-AP

Synonyme

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

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  WB analysis using 18138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18138-1-AP

  WB result of ABCD1 antibody (18138-1-AP, 1:600) with si-Control and si-ABCD1 transfected HeLa cells..

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  WB analysis of HepG2 using 18138-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18138-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18138-1-AP

  IP result of anti-ABCD1 (IP:18138-1-AP, 4ug; Detection:18138-1-AP 1:700) with HeLa cells lysate 3000 ug.

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  IHC staining of human ovary cancer using 18138-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 18138-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18138-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ABCD1 antibody (18138-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HeLa using 18138-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ABCD1 (18138-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

18138-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA ABCD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABCD1 fusion protein Ag12757
• Vollständiger Name: ATP-binding cassette, sub-family D (ALD), member 1
• Berechnetes Molekulargewicht: 745 aa, 83 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC025358
• Gene symbol: ABCD1
• Gene ID (NCBI): 215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Publikationen

Species	Application	Title
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
human	WB	Signal Transduct Target Ther; Structural insights into substrate recognition and translocation of human peroxisomal ABC transporter ALDP; Authors - Chao Xiong
human	WB	Leukemia; Targeting ABCD1-ACOX1-MET/IGF1R axis suppresses multiple myeloma; Authors - Zhannan Han